ISSN:
1617-4623
Schlagwort(e):
Ribosomal protein
;
secY
;
Protein secretion
;
Gene disruption
;
rpmJ
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary A unique feature of the spc ribosomal protein operon is that its region distal to the promoter contains a gene (secY) for an integral membrane protein, followed by an open reading frame termed X which has recently been proposed to encode a new ribosomal protein (protein B). We now show that the open reading frame X indeed directs the synthesis of a protein with electrophoretic mobilities similar to the B protein, and this supports the proposal that X may be more appropriately called rpmJ. Insertion of a plasmid sequence into the secY-rpmJ boundary of the chromosome caused a reduced expression of secY probably by destabilizing the secY part of the message. The results of complementation experiments suggested that a normal level of expression of rpmJ is not required for growth or protein secretion.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00330934
Permalink
