ISSN:
1435-1463
Keywords:
Human
;
serotonin transporter
;
cDNA sequence
;
polymerase chain reaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A cDNA encoding a serotonin transporter (5-HTT) in the human dorsal raphe nucleus was isolated and sequenced using cross-species amplification of human 5-HTT partial cDNA by the polymerase chain reaction (PCR) and the RACE-PCR procedure, designed for rapid amplification of 3′ and 5′ cDNA ends. The cDNA contains an open reading frame encoding a hydrophobic polypeptide of 630 amino acids with a calculated molecular weight of ∼ 70 kDa. The human 5-HTT is ∼ 92% homologous to the rat protein but contains an additional consensus phosphorylation site for cAMP-dependent protein kinase recognition located in the cytoplasmic N-terminal region, while a potential protein kinase C phosphorylation site identified in the rat homolog is not conserved in the human 5-HTT. Hydropathicity analysis revealed twelve membrane spanning segments, a topology proposed for other cloned sodium-dependent transporters.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01244919
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