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  • 1
    Electronic Resource
    Electronic Resource
    The role of nickel on somatic embryogenesis in Setaria italica L. in vitro (1998)
    Springer
    Euphytica 101 (1998), S. 319-324 
    Details
    ISSN: 1573-5060
    Keywords: nickel ; somatic embryogenesis ; tolerant cell lines ; in vitro ; Setaria italica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nickel (0.13, 0.25, 0.5, 1.0 and 1.5 mg/l) increased the efficiency of somatic embryogenesis in leaf base and mesocotyl derived calli of Setaria italica. A lower concentration of nickel in the culture media promoted long-term maintenance of embryogenic calli that regenerated into plantlets. The plants obtained from embryogenic calli grown on Ni-containing medium showed tolerance to nickel. The growth of embryogenic callus was the maximum at 1.5 mg/l as compared to a lower or a higher concentration of Ni. Use of nickel may help in the induction of high frequency somatic embryogenesis in Setaria italica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018386912104
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    in Vitro Somatic Embryogenesis in Typhonium Trilobatum Schott. (1999)
    Springer
    Plant growth regulation 27 (1999), S. 195-199 
    Details
    ISSN: 1573-5087
    Keywords: growth regulator ; in vitro ; medicinal plant ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An in vitro protocol was developed for the production of plants via somatic embryogenesis in callus cultures derived from petiole and leaf explants of Typhonium trilobatum. Optimum callus formation was achieved on semisolid Murashige and Skoog's [9] medium supplemented with 0.25 mg L−1 kinetin and 3.0 mgL−1 1-naphthaleneacetic acid (NAA) after 6 weeks of culture. Somatic embryogenesis was achieved upon transferring the callus to a medium containing 1.0 mg Lminus 1 kinetin and 0.25 mg Lminus 1 NAA. In vitro tuberization was also achieved on medium containing 1/2 strength MS basal salts supplemented with 1.0 mg L−1 Kinetin and 0.1 mg L−1 NAA. Embryo maturation and germination was achieved on the MS basal salts supplemented with 0.01 mg L−1 NAA and 2% (w/v) sucrose. Some thousands somatic embryo derived plantlets were hardened in the greenhouse and eventually planted in the open field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006278716645
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in callus cultures of Rosa hybrida L. cv. Landora (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 65-69 
    Details
    ISSN: 1573-5044
    Keywords: callus ; in vitro ; L-proline ; Rosa hybrida cv. Landora ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus was initiated from immature leaf and stem segments of rose (Rosa hybrida cv. Landora) and subcultured every four weeks on a basal medium of half-strength Murashige & Skoog (1962) salts plus 30 g l-1 sucrose (1/2 MS) and supplemented with 2.2 μM BA, 5.4 μM NAA and 2.2–9.0 μM 2,4-D. Embryogenic callus and subsequently somatic embryos were obtained from 8-week-old callus culture on 1/2 MS+2.2 μM BA+0.05 μM NAA+0.3 μM GA3+200−800 mg l-1 L-proline. Long-term cultures were established and maintained for up to 16 months by repeated subculture of embryogenic callus on L-proline deficient medium. About 12% of cotyledonary stage embryos taken from cultures cold-stored at 8±1°C for 4 days germinated on 1/2 MS+2.2 μM BA+0.3 μM GA3+24.7 μM adenine sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048208
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    Paper (German National Licenses)
    Fulltext
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