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  • 1
    ISSN: 1573-5060
    Keywords: breeding ; dormancy ; gibberellic acid ; in vivo culture ; scarified seed ; Helianthus annuus ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The importance of developing new techniques that would simplify the complexity and operative costs of genetic breeding plans has been widely recognized. In that sense, germination from immature seeds in sunflower is a simpler and cheaper alternative to that of in vitro embryo culture, as it is produced in non-sterile conditions. Different techniques to maximize germination percentage were tested using 15 day old seeds. The results indicate that it was important: 1) to scarify seeds and 2) to soak them in 100 ppm of gibberellic acid for 1 hour. Through the application of this technique more than 94% of the seeds germinated.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: breeding ; Helianthus annuus ; principal component analysis ; resistance test ; Sclerotinia sclerotiorum ; sunflower ; white rot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty sunflower inbred lines were studied for their reactions to 7 Sclerotinia sclerotiorum tests on different plant parts. A principal component analysis (P.C.A.) and t-tests on the means of resistant and susceptible groups indicated that reactions of inbred lines to infection of capitula by ascospores are independent from those to ascospore infections of terminal buds. They are even more contrasted with the results of any test measuring mycelial extension. However, for the last, there is a close association between the reactions of roots, leaves and capitula. Of the inbred lines, some showed good levels of resistance to most forms of attack, others were generally susceptible and there were some with good resistance to one particular form of attack. A P.C.A. of 18 morphological and field characters showed no general association between these characters and Sclerotinia sclerotiorum test results, although it was found that lines resistant to mycelial extension on capitula were generally earlier than those that were more susceptible. It is proposed that breeding programmes for general resistance to Sclerotinia sclerotiorum should include a combination of two or three tests.
    Type of Medium: Electronic Resource
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