ISSN:
1573-8221
Keywords:
superoxide dismutase
;
erythrocytes
;
platelets
;
lymphocytes
;
granulocytes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The level of superoxide dismutase (SOD) activity calculated relative to protein in the various human blood cells falls in the following order: platelet 〉 erythrocyte 〉 lymphocyte 〉 granulocyte. During electrophoresis in homogeneous polyacrylamide gel of homogenates of granulocytes, lymphocytes, and platelets three zones of SOD activity were identified. Two fractions of the enzyme, disappearing after treatment with cyanide, were found in lysates of erythrocytes after removal of the hemoglobin. Of the two SOD fractions of platelets, lymphocytes, and granulocytes migrating rapidly toward the anode, the first corresponds in its ability to be inhibited by cyanide or organic solvents to the cytosol isozyme, the second to the mitochondrial isozyme. The third cathode fraction was not identified. The functional role of SOD in specialized blood cells and the cause of heterogeneity of the enzyme are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00799193
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