ISSN:
1573-2568
Keywords:
ulcerative colitis
;
HLA
;
pathogenesis
;
susceptibility
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract HLA-DRB1 allele typing was performed by the PCR-RFLP method on 59 ulcerative colitis (UC) patients and 136 healthy controls. Phenotypic frequencies of HLA-B52 and DR2 were significantly increased among the UC patients, serologically. DNA typing of HLA-DRB1 revealed that the genotypic frequency of DRB1*1502 was higher in UC than in the controls (49.2% vs 17.6%;P〈0.0001). In the analysis of clinical parameters, 82.8% of patients bearing DRB1*1502 were treated with corticosteroids. DRB1*1501 and DRB1*1502 differ in only one amino acid at residue 86 (valine vs glycine), and 66% of the UC patients carried two glycines at position 86 in the HLA-DRβ-chain (vs 51% of control;P〈0.05). These observations suggest that the presence of Gly-86 in the HLAβ-chain and surrounding amino acid sequence of HLA-DRB1*1502 is strongly associated with susceptibility to UC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02064985
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