ISSN:
1573-4978
Keywords:
acute-phase
;
haptoglobin gene
;
HMG-1 protein
;
phosphorylation
;
rat liver
;
transcription factor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A 29 kD soluble rat liver nucleoprotein (p29) has increased binding affinity for the hormone responsive element (RE) of the rat haptoglobin (Hp) gene during the acute-phase reaction. In this work the possibility of its structural and functional homology to the high mobility group 1 (HMG1) nonhistone protein constituent of chromatin was examined. The results of two-dimensional gel electrophoresis, Southwestern and Western immunoblot analyses, showed that p29 and HMG1 are homologous protein species. On the basis of in vitro and in vivo phosphorylation/dephosphorylation experiments, we discuss the modulatory role of phosphate groups in view of the structure and function of p29.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00424433
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