ISSN:
1075-2617
Schlagwort(e):
biosynthesis
;
carbon-13
;
nitrogen-15
;
serine hydroxymethyltransferase
;
tryptophan synthase
;
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
L-[1,2-13C2,15N]Serine was prepared from [1,2-13C2,15N]glycine on a gram scale by the use of the enzyme serine hydroxymethyltransferase. The reaction was monitored by 13C-NMR spectroscopy. This is the first simultaneously 13C- and 15N-labelled serine isotopomer so far reported. Part of the product was directly converted by tryptophan synthase to L-[1,2-13C2,15N]tryptophan which could conveniently be purified and isolated as Boc-derivative in a yield of 71%. Most of the serine was isolated similarly but to remove remaining starting material in this case purification by column chromatography was required. © 1997 European Peptide Society and John Wiley & Sons, Ltd.J. Pep. Sci.3: 361-366No. of Figures: 1. No. of Tables: 0. No. of References: 32
Zusätzliches Material:
1 Ill.
Materialart:
Digitale Medien
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