ISSN:
1573-6903
Keywords:
Affinity HPLC
;
Thymosin β4
;
cAMP
;
calmodulin
;
phosphodiesterase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A fragment (11–19) of thymosin β4 was found to stimulate phosphodiesterase activity even in the absence of calcium and calmodulin. Half-maximal enzyme activation occurred with 10 nM peptide, and was further increased by phospholipids such as phosphatidylserine. The mechanism of stimulation is an increase in the Vmax of cAMP degradation without a substantial change in the Km for the substrate. In the presence of calcium ions and calmodulin the peptide was also stimulatory.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00967323
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