Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
The @journal of eukaryotic microbiology
13 (1966), S. 0
ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
SYNOPSIS. Five- to 6-day-old resting cells of Ochromonas malhamensis were incubated at pH 6.5 with glucose and appropriate C14 precursors of the methyl groups of phospholipid-choline. Under the experimental conditions L-methionine-C14H3 was the most efficient source of choline-methyl groups, followed by formate-C14, formaldehyde-C14 and DL-serine-3-C14, respectively. Glycine-2-C14 was not incorporated into choline. Both L-methionine-C14H3 and formate-C14 served as precursors for the methyl groups of monomethylethanolamine, dimethylethanolamine and choline. Addition of non-radio-active L-methionine depressed the incorporation of formate-C14 into choline-methyl groups by 50%. The results support the hypothesis that methionine can be the source of all 3 methyl groups of choline, and that formate is probably converted to the methyl group of methionine before transmethylation to choline. However, an alternate pathway from single-carbon sources cannot be excluded.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1966.tb01882.x
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