ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Single cell suspensions were prepared from the choroids of adult rabbit eyes using Pronase digestion, gentle pipetting and centrifugation. When diluted and inoculated into medium F12 supplemented with 5% fetal bovine serum and 10% rabbit serum, the choroid cell suspensions yielded large, healthy colonies with an average plating efficiency of about 45%. The growth in culture of a large portion of the cells from a normal non-embryonic tissue opens new possibilities for the study of cellular differentiation in vitro.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040700307
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