ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A simple and reliable technique was developed to prepare pure monoclonal antibody (MAb) to interleukin-2 using cells entrapped in novel composite poly(N-vinyl caprolactam)-calcium alginate beads. Flow cytometry was applied to study cell size and cell cytoplasm granularity distribution. Maximum MAb production by the gel-entrapped cells in serum free medium was 2-3-fold higher compared to free suspension culture in serum containing medium. The only contaminating protein in culture supernatant was transferrin at 5% w/v.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008839122591
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