Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    EFFECT OF INSULIN ON LEVELS AND TURNOVER OF INTERMEDIATES OF BRAIN CARBOHYDRATE METABOLISM IN VIVO (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 18 (1971), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— –The rates of incorporation of 14C from [U-l4C]glucose into intermediary metabolites have been measured in rat brain in vivo. The time course of labelling of glycogen was similar to that of glutamate and of glucose, which were all maximally labelled between 20 and 40min, but different from lactate, which lost radioactivity rapidly after 20min. The extent of labelling of glycogen (d.p.m./μmol of glucose) was of the same order as that of glutamate at 20 and 40 min after injection of [14C]glucose. However, calculations of turnover rates showed that glutamate turns over some 8-10 times faster than glycogen. Insulin, intracisternally applied, produced after 4-5 h a 60 per cent increase in glucose-6-P and a 50 per cent increase in glycogen. There was no change in the levels of glucose, glutamate or lactate, nor in the activity or properties of the particulate and soluble hexokinase of the brain. The injection of insulin affected neither the glycogen nor glucose contents of skeletal muscle from the same animals. The effects of insulin on the incorporation of l4C into the metabolites contrasted with its effects on their levels. The specific activities of glycogen and glucose were unchanged and there was a slight but non-significant increase in the specific activity of glutamate. The time course of incorporation into lactate was unaffected up to 20 min, but a significant delay in the loss of 14C after 20 min occurred as a result of the insulin injection. At 40 min, the specific activity of cerebral lactate was 60 per cent higher in insulin-treated animals than in control animals. The results are interpreted in terms of an effect of insulin on glucose uptake to the brain, with possibly an additional effect on a subsequent stage in metabolism, which involves lactate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb09585.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    RATES OF CEREBRAL GLUCOSE UTILIZATION IN RATS ANAESTHETIZED WITH PHENOBARBITONE (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 20 (1973), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— 〈list xml:id="l1" style="custom"〉1Intraperitoneal injection of phenobarbitone (250 mg/kg body wt.) into rats caused increased brain concentrations of glucose (100 per cent), glucose 6-phosphate (16 per cent) and ATP (12 per cent) and decreased concentrations of lactate (33 per cent) and ADP (15 per cent). A 31 per cent decrease in glutamate content was not statistically significant. No significant change occurred in the cerebral contents of glycogen or creatine phosphate.1The rates of increase in the brain of specific activities, in the first few minutes after systemic injection of [U-14C]glucose, of glucose, lactate, glutamate and glycogen were all halved by phenobarbitone. Calculated flux rates of 14C from glucose into metabolic intermediates and from lactate to glutamate were also decreased by 27–47 per cent; the effects on rate constants showed inconsistencies. The rate constants for conversion of glucose to lactate and to glutamate were decreased by 60–70 per cent, but that from lactate to glutamate was virtually unchanged. The rate constant for the flux from glucose to glycogen was reduced by 39 per cent, but the accumulation of glucose meant that the actual flux into glycogen increased by 20 per cent.1The results are interpreted in terms of an effect of the barbiturate not only on glucose transport, but also at an enzymic stage in glycolysis, possibly hexokinase or phosphofructokinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1973.tb00069.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A COMPARISON OF SOME ASPECTS OF THE PHYSIOLOGY AND METABOLISM OF THE NERVOUS SYSTEM OF THE LOCUST SCHISTOCERCA GREGARIA IN VITRO WITH THOSE IN VIVO (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 31 (1978), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Studies were made on isolated ganglia of the locust in a modified O2 electrode. The basic requirements of a suitable incubation medium were determined and found to include saturation of the medium with 100% O2, and 10mm-glucose as energy source. The effects of incubation in vitro were determined for a number of biochemical characteristics including O2 uptake and the concentrations of Arg P, ATP, ADP, glycogen, glucose, lactate and pyruvate. Action potentials were recorded from a major nerve both in vivo and in vitro. On the basis of these biochemical and physiological studies, it was concluded that the tissue functions well in vitro. Some comparisons were made between the metabolic characteristics found in the locust nervous system, and those of mammalian tissue. The locust ganglia were found to have a rate of O2 uptake much higher than comparable mammalian tissue, and to contain a considerable reserve of glycogen. The low ratio of lactate/pyruvate suggests a more aerobic metabolism than is the case in mammalian brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1978.tb12441.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A METHOD FOR THE ISOLATION AND ESTIMATION OF GABA, GLUTAMATE AND GLUTAMINE FROM COMPLEX MIXTURES BY A COMBINATION OF ENZYMIC INTERCONVERSION AND FORMATION OF 1-DIMETHYLAMINONAPHTHALENE-5-SULPHONYLBUTYROLACTAM (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 25 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— A method is presented which allows the isolation and estimation of GABA, glutamate and glutamine in extracts of nervous tissue. It depends on the enzymic conversion of the last two compounds to GABA, before isolating the GABA as DNS-γ-butyrolactam. The low polarity of this compound makes this possible without the use of chromatography. Furthermore, the specific radioactivities of these 3 compounds in a complex mixture may be found without elaborate separation procedures. Details are given of optimum reaction conditions, and comparisons made with other methods of estimation of these amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb07689.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    EXTRACTION, PURIFICATION AND TURNOVER OF RAT BRAIN GLYCOGEN (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 18 (1971), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Glycogen was prepared from rapidly frozen rat brain by the usual techniques and found to contain considerable amounts of non-glycogen carbohydrate. The crude glycogen was partially purified by extraction with hot or cold water and reprecipitation. Enzymic estimation showed that the carbohydrate extracted into hot water contained only 50 per cent of glucose after hydrolysis; of the hot water insoluble material, namely some 30 per cent of the total carbohydrate present in the crude glycogen, less than half of the carbohydrate was released by hydrolysis in 1 M-HC1. The glycogen soluble in hot water incorporated 14C from [14C]glucose at considerably higher rates than the residual material and also decreased more rapidly during post-mortem autolysis. Glycogen extracted into cold water was of higher purity than that extracted by hot water; although the material behaved as glycogen during precipitation and re-extraction it contained only 75 per cent of its carbohydrate as glucose. Contaminants included fucose, galactose and hexuronic acid. The rates of metabolism of the partially purified glycogen are compared with published rates; it is suggested that the observed rates are inaccurate due to the impurities present in brain glycogen prepared by classical techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb12035.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Behavior and fate of two phenylpyridazinone herbicides in cotton, corn, and soybean (1974)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 22 (1974), S. 1119-1125 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf60196a051
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    A comparison of laser Doppler flowmetry with other methods of assessing the vitality of traumatised anterior teeth (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Dental traumatology 15 (1999), S. 0 
    Details
    ISSN: 1600-0595
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Evans D, ReidJ, Strang R, Stirrups D. A comparison of laser Doppler flowmetry with other methods of assessing the vitality of traumatised anterior teeth. Endod Dent Traumatol 1999; 15: 284–290. © Munksgaard, 1999.Abstract — Laser Doppler flowmetry is a non-invasive electro-optical technique which allows the semi-quantitative recording of pulpal blood flow. This study aimed to determine the reliability (measured as the sensitivity and specificity) of laser Doppler flowmetry as a method of assessing the vitality of traumatised anterior teeth, and to compare it with standard pulpal diagnostic tests. Recordings of pulpal blood flow were taken from 67 non-vital anterior teeth (55 patients), where the pulpal status was confirmed by pulpectomy. For comparison, recordings were also taken from 84 vital anterior teeth (84 patients). Analysis of the recordings allowed diagnostic criteria to be developed which gave the technique a sensitivity and specificity of 1.0 for this sample. None of the other standard pulpal diagnostic methods tested was as reliable. This was usually due to low sensitivities, which ranged between 0.92 for sensibility testing with ethyl chloride down to 0.36 for periapical radio-lucency and 0.16 for a history of pain. Laser Doppler flowmetry was found to be a reliable method of assessing the pulpal status of traumatised anterior teeth, although it is technique-sensitive and time-consuming to use.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-9657.1999.tb00789.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Condition factor and hepatosomatic index as estimates of energy status in male three-spined stickleback (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seasonal variation in direct and indirect measures of energy status was examined using estimates of glycogen, lipid and protein levels in a single cohort of male three-spined sticklebacks from an annual population collected each month over one complete year. Condition factor, somatic condition factor and hepatosomatic index (HSI) were calculated as indirect indices of energy status and the accuracy of these indirect measures as predictors of energy status was investigated. Results indicate that both condition factors were significant predictors of energy reserves (lipid, protein, glycogen and total energy), but that the proportion of variance accounted for was small. Both condition factors perform better as predictors of energy content per unit body weight. The HSI was a significant, but a weak predictor of total glycogen levels over the whole year. On a seasonal basis the relationship between HSI and energy reserves was highly variable. These indices are therefore poor predictors of energy reserves in male three-spined sticklebacks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1995.tb06002.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Annual variation in energy reserves in male three-spined stickleback, Gasterosteus aculeatm L. (Pisces, Gasterosteidae) (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 35 (1989), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Wet and dry weights of tissue were measured and concentrations of glycogen, lipid and protein were estimated for the liver, gonad and carcass of male sticklebacks from an annual population collected each month over one complete year. In young-of-the-year there is one period of rapid weight gain, in all three body regions (liver, carcass and gonads) but particularly of the carcass, in the autumn and a second in spring and early summer. This is accompanied by an increase in the water content of all three body regions. The gonadosomatic index also increases sharply in spring and early summer. Young sticklebacks accumulate lipid and glycogen slowly during the autumn and winter of their first year of life and more rapidly from late winter to early summer. Thus, the period of most rapid accumulation of these reserves coincides with the time when body weight and gonad maturation are also increasing sharply. Lipid and glycogen levels fall during the reproductive season in those males that breed, so that by July they are reduced to 43% and 37% (respectively) of their peak values in May. Levels of protein increase throughout the year as the fish grow, but in breeding males by July the concentration of protein in the carcass falls to 70% of pre-breeding levels. Breeding males therefore reach the end of the reproductive season with their total energy reserves severely reduced, and consequently they suffer a very high mortality. In contrast, adult males that fail to reproduce survive beyond the breeding season. They continue to gain weight and to accumulate lipid and glycogen from August to September, but these energy reserves fall (to levels comparable to those of post-breeding fish) in December, when these fish probably die. These results demonstrate that in male sticklebacks, growth and gonad maturation can be sustained in parallel with the accumulation of energy reserves, which are subsequently extensively depleted as a result of reproductive activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1989.tb02976.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Energy reserves and reproductive investment in male three-spined sticklebacks, Gasterosteus aculeatus (2001)
    Copenhagen : Munksgaard International Publishers
    Ecology of freshwater fish 10 (2001), S. 0 
    Details
    ISSN: 1600-0633
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract – In a 6-month study of male sticklebacks from a single cohort of an annual population, energy reserves (carbohydrate, lipid and protein) in the carcass and gonad were found to increase from December to May. Energy reserves in the liver increased from December to March before declining between March and April, a period of marked gonadal growth in this population. Examination at the individual level of the interrelationships among the various components of the energy store (corrected for differences in body size) identified overall body condition as a major component of variation and highlighted a negative relationship between energetic investment in gonadal tissue and energy reserves in various somatic compartments. Early in the season, relative gonadal weight was largely unrelated to somatic energy reserves, but from March onwards there was a negative correlation between these two variables. These data suggest that a significant flux of glycogen and lipid from the somatic to the gonadal tissue occurs in male sticklebacks in the period just prior to the breeding season, with the extent of gonadal growth varying between individuals and occurring at the expense of overall nutritional status./〉
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0633.2001.100206.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...