ISSN:
1573-2576
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract On immunoblots of human neutrophil cytoplasts (U-CYT), a previously undescribed 97kDa protein was revealed by intense and selective reaction with an antibody that was initially raised to recognize PKC-γ. Denoted “γ-rp” for gamma-related protein, this acidic cytosolic protein somewhat resembled the classic forms of PKC in several biochemical respects. Appearing as a doublet on low-percentage SDS-PAGE gels, both its mobility and staining pattern were rapidly altered by treatment of U-CYT with either phorbol ester or chemotactic peptide. Whole neutrophil γ-rp was detectable only after TCA precipitation of intact cells. It was also detectable in human platelets, lymphocytes, and neutrophil-like differentiated HL60 cells, but not in fibroblasts, erythrocytes, monocytes, or monocyte-like differentiated HL60 cells. Our data suggest that γ-rp merits further study as a potential participant in cellular activation, and as a possible structural or functional relative of PKC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022390406225
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