ISSN:
1573-5001
Keywords:
Dihydrofolate reductase
;
NMR
;
13C/15N labelled protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary 13C-based three-dimensional 1H−1H correlation experiments have been used to determine essentially complete 13C and 1H resonance assignments for the amino acid side chains of uniformly 13C/15N labelled L. casei dihydrofolate reductase in a complex with the drug methotrexate. Excellent agreement is observed between these assignments and an earlier set of partial assignments made on the basis of correlating nuclear Overhauser effect and crystal structure data, indicating that the tertiary structure of the enzyme is similar in solution and in the crystal state.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00174608
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