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  • 11
    Electronic Resource
    Electronic Resource
    An Electron Microscopical Study of the Interaction of Monoclonal Antibodies with Gametes of the Malarial Parasite Plasmodium gallinaceum (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Anti-malarial gamete antibodies prevent the fertilization of gametes in the mosquito midgut and prevent transmission of malaria. Recently, hybridoma cell lines secreting monoclonal antibodies (10G3 and 11C7) against gametes of the malarial parasite have been developed. These antibodies act synergistically to mediate 80–90% suppression of the infectivity of gametocytes, although neither monoclonal antibody alone has a significant effect on gametocyte infectivity. We performed immuno-electron microscopy to characterize the interactions of these monoclonal antibodies with gametes of Plasmodium gallinaceum. Male gametes exposed to either 10G3 or 11C7 agglutinated into loose clusters, while those exposed to a mixture of 10G3 and 11C7 agglutinated into long, rope-like bundles. This difference appears to be related to the distribution of the antibodies on the surface of the gametes. When 10G3 or 11C7 labeled with a ferritin-conjugated anti-mouse Ig were used singly, the ferritin particles were distributed in focal areas over the surface of the parasites. By contrast, when the male gametes were exposed to a mixture of 10G3 and 11C7, the ferritin particles were distributed over their entire surface. Female gametes reacted similarly to these antibodies. These observations indicate that combinations of antibody specificities that reduce fertilization efficiency coat the entire surface of the gametes. On the other hand, focal interactions resulting from a single antibody are unable to block fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02871.x
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  • 12
    Electronic Resource
    Electronic Resource
    Plasmodium falciparum protein associated with the invasion junction contains a conserved oxidoreductase domain (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 15 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The merozoite cap protein-1 (MCP-1) of Plasmodium falciparum follows the distribution of the moving Junction during invasion of erythrocytes. We have cloned the gene encoding this protein from a cDNA library using a monoclonal antibody. The protein lacks a signal sequence and has no predicted trans-membrane domains; none of the antisera reacts with the surfaces of intact merozoites, indicating that the cap distribution is submembranous. MCP-1 is divided into three domains. The N-terminal domain includes a 52-amino-acid region that is highly conserved in a large family of bacterial and eukaryotic proteins. Based on the known functions of two proteins of this family and the pattern of amino acid conservation, it is predicted that this domain may possess oxido-reductase activity, since the active cysteine residue of this domain is invariant in all proteins of the family. The other two domains of MCP-1 are not found in any other members of this protein family and may reflect the specific function of MCP-1 in invasion. The middle domain is negatively charged and enriched in glutamate; the C-terminal domain is positively charged and enriched in lysine. By virtue of its positive charge, the C-terminal domain resembles domains in some cytoskeleton-associated proteins and may mediate the interaction of MCP-1 with cytoskeleton in Plasmodium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.tb02260.x
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  • 13
    Electronic Resource
    Electronic Resource
    Predicting mortality in patients with malarial acute renal failure (2000)
    Melbourne, Australia : Blackwell Science Pty
    Nephrology 5 (2000), S. 0 
    Details
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Acute Physiology and Chronic Health Evaluation (APACHE) III scores, calculated within the first 24 h of admission, were analysed in 108 patients with acute renal failure due to falciparum malaria who were admitted to Bangkok Hospital for Tropical Diseases, Thailand. Twelve (11.1%) patients died. The mean APACHE III score was 82.0 ± 25.5 (range, 45–171). There was a close relation between the APACHE III score and the hospital mortality rate. The non-survivors had significantly higher APACHE III scores than the survivors, 109.8 ± 36.7 and 75.7 ± 21.6, respectively (P 〈 0.001). Patients with APACHE III score ≥ 82 had a 4.2-fold higher risk of dying compared with patients with a lower score (95% CI 1.2–14.7; P = 0.013). Haemodialysis treatment was performed in 97 (89.8%) of the patients. The mean APACHE III score for patients who were not treated with haemodialysis (95.9 ± 38.0) was not significantly higher than those who received haemodialysis (80.4 ± 23.5; P 〉 0.05), but the former had a 4.4-times higher risk of dying compared with those dialysed (95% CI 1.6–12.3; P = 0.019). Using the APACHE III score and its ability to predict death, we calculated its sensitivity, specificity and accuracy to be 0.92, 0.31 and 0.41, respectively, at a cut-off score of 67 points. The area under the receiver operating characteristic (ROC) curve was 0.75. The APACHE III scoring system correlated well with the outcome of critically ill malaria patients with acute renal failure, although it was not possible to identify individual survivors or non-survivors. APACHE III should not be used for individual prognosis or treatment decisions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1440-1797.2000.00505.x
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  • 14
    Electronic Resource
    Electronic Resource
    An Electron Microscope-Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites (1973)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 20 (1973), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Limiting membranes of malaria parasites and host red cells stain differently when exposed to positively charged iron colloid. Negatively charged red cell membranes avidly bind colloid, whereas parasite membranes do not. This selectivity in colloidal iron uptake by the 2 types of membranes can be utilized as an aid in discerning the amounts of contaminating host cell membranes in “free” malaria parasite preparations and in related cell-free membrane extracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1973.tb03583.x
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  • 15
    Electronic Resource
    Electronic Resource
    A Comparative Study of Gametocyte Ultrastructure in Avian Haemosporidia (1973)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 20 (1973), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The fine structure of gametocytes of 3 avian haemosporidian parasites Plasmodium gallinaceum, Haemoproteus columbae, and Leucocytozoon simondi has been studied and compared by electron microscopy. The gametocytes of all 3 species are bounded by a 3-layered limiting membrane system, possess a cytostome during some portion of their residence within host cells, and their sex can be distinguished by both nuclear and cytoplasmic characteristics. L. simondi differs most significantly from P. gallinaceum and H. columbae in possessing large intranuclear granules, mitochondria associated with pocket infoldings of the nuclear envelope near the atypical centriole complex and compartmentalization of the cytoplasm by segments of closely aligned unit membranes. Further, the cytostome of L. simondi does not appear to be a persistent structure as in the other 2 species and pigment is not present within food vacuoles. L. simondi also is capable of infecting a wider variety of host cells and within leukocytes produces striations of the host nucleus and an apparent spiral banding of the host cell surface. The comparison of P. gallinaceum, H. columbae, and L. simondi gametocytes by electron microscopy leads to the conclusion that Plasmodium and Haemoproteus are more closely related to each other than either of them is on Leucocytozoon.The terminology used to describe certain organelles within the gametocyte's cytoplasm has been reexamined and the relationship of the nucleolus to parasite maturation also is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1973.tb06008.x
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  • 16
    Electronic Resource
    Electronic Resource
    Cytoadherence of knobless Plasmodium falciparum -infected erythrocytes and its inhibition by a human monoclonal antibody (1989)
    [s.l.] : Nature Publishing Group
    Nature 338 (1989), S. 763-765 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A bulk culture of the Palo Alto (PA) strain of P. falciparum bound poorly to the melanoma cell line C32 (100-200 infected red blood cells (RBC) per 100 melanoma cells). However, the number of binding RBC was significantly increased (2,000-3,000 RBC per 100 melanoma cells) after four consecutive ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/338763a0
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  • 17
    Electronic Resource
    Electronic Resource
    Paramyosin and actin in schistosomal teguments (1988)
    [s.l.] : Nature Publishing Group
    Nature 333 (1988), S. 76-78 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Diagrammatic representation of the adult schistosome tegument and underlying structures, bl, basal lamina; cm, circularly arranged muscle fibres; ct, cytoplasmic tube; eb, elongate body; g, Golgi complex; hm, heptalaminate outer membrane; 1m, longitudinal muscle fibres; mb, membranous body; ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/333076a0
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  • 18
    Electronic Resource
    Electronic Resource
    High voltage electron microscopy on microgametogenesis of Haemoproteus columbae (1974)
    Springer
    Cell & tissue research 147 (1974), S. 353-360 
    Details
    ISSN: 1432-0878
    Keywords: High voltage electron microscopy ; Microgametogenesis ; H. columbae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The microgametogenesis of Haemoproteus columbae was investigated by high voltage and conventional electron microscopy and the results were compared. The features of microgametogenesis which have become clear by high voltage electron microscopy include the distribution and arrangement of axonemes in the microgametocyte and the budding process and structure of the microgametes. High voltage electron microscopy shows that prior to exflagellation the axonemes occupy a major portion of the microgametocyte cytoplasm, and are arranged in an orderly fashion. Conventional electron microscopy suggests that the axonemes are randomly distributed in the microgametocyte cytoplasm. High voltage electron microscopy shows that a short portion of the axoneme protrudes outward in the early stage of microgamete budding, while the major portion remains within the cytoplasm. The microgamete of H. columbae as observed by the high voltage electron microscope, is composed of a slender nucleus and an axoneme which run along the same axis and spiral one another. The nucleus is slightly bulbous at the anterior end and at the midportion of the microgamete. These findings have not been obtained with certainty by conventional electron microscopy because the thin section technique could not provide a sufficient profile of the long wavy microgamete.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307470
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  • 19
    Electronic Resource
    Electronic Resource
    Junctional apparatus in erythrocytes infected with malarial parasites (1972)
    Springer
    Cell & tissue research 124 (1972), S. 72-75 
    Details
    ISSN: 1432-0878
    Keywords: Erythrocytes ; Monkeys ; Malaria ; Junctional apparatus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of erythrocytes infected by the malarial parasite,Plasmodium falciparum, revealed electron dense, cone-shaped excrescences (∼40 mμ in height and ∼100 mμ in width) along the plasma membrane. These excrescences form focal junctions with the plasma membrane of reticular cells of the spleen. The resulting complexes appear to be responsible for sequestration of erythrocytes infected byP. falciparum in the spleen of the host.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00981942
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  • 20
    Electronic Resource
    Electronic Resource
    Junctional apparatus in erythrocytes infected with malarial parasites (1972)
    Springer
    Cell & tissue research 124 (1972), S. 72-75 
    Details
    ISSN: 1432-0878
    Keywords: Erythrocytes ; Monkeys ; Malaria ; Junctional apparatus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of erythrocytes infected by the malarial parasite, Plasmodium falciparum, revealed electron dense, cone-shaped excrescences (∼40 mμ in height and ∼100 mμ in width) along the plasma membrane. These excrescences form focal junctions with the plasma membrane of reticular cells of the spleen. The resulting complexes appear to be responsible for sequestration of erythrocytes infected by P. falciparum in the spleen of the host.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335455
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