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  • 11
    Electronic Resource
    Electronic Resource
    Etude histo-chimique d'un type de cellules alveolaires du poumon: Les cellules a inclusions lipidiques complexes (1968)
    Springer
    Histochemistry and cell biology 15 (1968), S. 21-37 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les cellules à inclusions phospholipidiques forment un contingent notable des cellules alveolaires du poumon. Leur richesse en phospholipides saturés traduirait ici un fait particulier, concernant le métabolisme lipidique du poumon normal. Elles se caractérisent morphologiquement par leur aspect, surtout en utilisant des méthodes histochimiques, après fixation bichroniatée; elles se signalent aussi par leur richesse en enzymes oxydatifs, déshydrogénases, cytochrome-oxydases. Elles contiennent en outre, des phosphatases alcalines et des estérases non spécifiques. Cette richesse enzymatique parait en rapport avec une activité métabolique importante. Leur modification dans les phénomènes pathologiques, bien que difficile à prèciser, semble intéressante. Ces éléments et les substances qu'ils élaborent, seraient en relation avec la formation du film endo-alvéolaire ou Surfactant. Néanmoins, des études plus précises, en particulier autoradiographiqes et histoenzymologiques ultrastructurales seraient nécessaires afin de corroborer et de compléter ces données.
    Notes: Summary Cells containing phospholipid inclusions represent a considerable contingent of the alveolar cells of the lung. Their high content in saturated phospholipids is a special feature of the metabolism of the normal lung. Morphologically they can be distinguished by their appearance; bichromate fixation followed by biochemical techniques is used for their determination. They also distinguish themselves by their abundance in oxidative enzymes, dehydrogenases and oxidases. They also contain alkaline phosphatases and non-specific esterases. The abundance of enzymes seems to indicate that they play a very active part in metabolism. The way the cells change during pathological processes although this change is difficult to definepresents an interesting problem. The cells themselves and the substances which they secrete are connected with the formation of the endo-alveolar lining. It will, however, be necessary to do more work, particularly autoradiographic and histo-enzymatic ultrastructural studies, in order to confirm and to complete these findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304571
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  • 12
    Electronic Resource
    Electronic Resource
    Aspect ultrastructural des lesions induites au niveau du poumon de souris par le N-nitroso-N-methylurethane (1972)
    Springer
    Journal of cancer research and clinical oncology 77 (1972), S. 279-291 
    Details
    ISSN: 1432-1335
    Keywords: Lung mouse tumors ; Chemical carcinogen ; Ultrastructure ; Pneumocyte II ; Crystalline inclusions ; Interstitial fibrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The pulmonary tumours induced in Swiss mice by NMUR seem to be constituted for the most part by cells closely related to the granular pneumocytes (pneumocytes II ou B). Other rarer cells, of epithelial bronchiolar aspect, may take part in the proliferation. The ultrastructural study of tumoral cells suggest that their secretory functions are disturbed. The origin of the crystalline intratumoral structures is discussed, as well as their possible relationship with the production of surfactant. The peritumoral areas are characterized by a dense interstitial flbrosis in which the collagen fibers are associated with an elastoid substance.
    Notes: Resumé Les tumeurs pulmonaires induites chez la souris swiss par le NMUR apparaissent essentiellement constituées de cellules apparentées aux pneumocytes granuleux (pneumocytes II ou B). D'autres cellules plus rares, de morphologie épithéliale bronchiolaire peuvent participer á la prolifération. L'étude ultrastructurale des cellules tumorales suggère que leurs fonctions secrétoires sont perturbées. Le mode de formation des structures cristallins intratumorales est discuté, ainsi que leur rapport éventuel avec la production du surfactant. Les territoires péri-tumoraux sont caractérisés par une fibrose interstitielle dense où les fibres de collagène sont associées à uns substance élastoïde.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283971
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  • 13
    Electronic Resource
    Electronic Resource
    Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level (1986)
    Springer
    Journal of molecular histology 18 (1986), S. 5-14 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The argyrophilic proteins of the nucleolar organizer region (Ag-NOR proteins) were specifically localized at the optical level with a modified one-step silver technique performed at 20° C. This method was applied to various materials including cells in smears, chromosomes, semi-thin sections of plastic-embedded cells and sections of paraffin-embedded human pathological tissues. In order to improve the visualization of the silver deposits we tested various modes of imaging, including bright-field, Nomarski contrast, reflected light and combined Nomarski contrast with reflected light. The use of Nomarski contrast is useful to define precisely the phases of mitosis. The use of reflected light, which is based on the ability of silver to reflect incident light specifically, gives images with an improved resolution compared to bright-field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01676192
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  • 14
    Electronic Resource
    Electronic Resource
    Canthaxanthin retinopathy (1987)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 225 (1987), S. 189-197 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The eyes of a patient with canthaxanthin retinopathy were obtained at autopsy and examined by light and electron microscopy. Various tissues of one eye were also studied by physicochemical methods. Morphologically, there were red, birefringent, lipid-soluble crystals in the inner layers of the entire retina. They were particularly large and numerous perifoveally, where they were also clinically visible, but they also occurred frequently in a ring-shaped form peripherally and, less frequently, equatorially. The crystals were located in a spongy degeneration of the inner neuropil, where atrophy of the inner parts of the Müller cells was noticed. The compound isolated from the retina was identical with synthetic canthaxanthin according to mass and proton-resonance spectroscopy. Quantitatively, the retina contained up to 42 μg canthaxanthin per gram of tissue besides a minor amount of other carotenoids. Of the other tissues of the eye, only the ciliary body contained measurable concentrations of canthaxanthin. From the great number and size of the crystals, on the one hand, and the relatively small amount of isolated canthaxanthin on the other, it was concluded that the crystals presumably represent a canthaxanthin-lipoprotein complex rather than pure canthaxanthin alone. Examination showed that clinically, only the central portion of the canthaxanthin thesaurismosis, where crystals are packed most densely, can be seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02175448
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  • 15
    Electronic Resource
    Electronic Resource
    Etude cytochimique en ultrastructure de l'alvéole pulmonaire du rat (1968)
    Springer
    Cell & tissue research 91 (1968), S. 478-495 
    Details
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les auteurs étudient par des techniques enzymatiques (phosphatases alcalines, phosphatases acides et adénosines triphosphatases) et des techniques autoradiographiques (par la glycine 3H), les cellules du revêtement de la paroi alvéolaire du rat Wistar. Après avoir rapporté les caractéristiques enzymatiques des différents types de cellules, les auteurs aboutissent à un certain nombre de conclusions concernant la cytophysiologie et le métabolisme des phospholipides au niveau du poumon. Cette étude qui fait suite à une étude histochimique et histo-enzymologique en microscopie optique, leur permet de penser que les pneumocytes granuleux ou cellules à inclusions lipidiques ou cellules alvéolaires de type II jouent un rôle particulier dans l'élaboration du surfactant. En effet, des inclusions lipidiques complexes ont été retrouvées dans les mitochondries; elles sont par ailleurs le siège d'une réaction positive par les déshydrogénases, elles incorporent précocement la glycine tritiée et possèdent une période constante de 55–56 Angströems. Les images de secrétion exocrine retrouvées permettent de penser que ces cellules synthétisent des phospholipides (au niveau des mitochondries) destinés à l'élaboration du surfactant. La positivité des A.T. Pases au niveau de leurs villosités traduit une activité de transfert énergétique au niveau des membranes. Il existerait donc deux types de figures lamellaires au niveau des cellules bordant l'alvéole: les unes sont situées dans les pneumocytes granuleux et ne sont pas positives aux phosphatases acides; elles sont surement à l'origine du surfactant. Les autres sans période caractéristique marquées par une réaction phosphatasique acide nette correspondent à du matériel phospholipidique phagocyté et sont situées dans les cellules alvéolaires ou endo-alvéolaires à activité macrophagique.
    Notes: Summary The alveolar cells of the rat have been studied autoradiographically (glycine 3 H) as well as with certain enzyme demonstrating methods (alkaline phosphatases, acid phosphatases and A.T. Pases). The enzymatic features of the different cell types are given and conclusions about the cytophysiology and the phospholipid metabolism in the lung are suggested. The authors think that the granular pneumocytes (or phospholipid inclusion cell or Type II alveolar cell) play a role in the “surfactant” elaboration. Complex lipid inclusion bodies are to be seen in the mitochondria, which are dehydrogenase positive, incorporate glycine 3H, and possess a constant structural period of 55–56 Å. The exocrine secretion figures suggest that these cells synthesize phospho-lipids in their mitochondria; these lipids are part of the “surfactant”. The positive A.T. Pases on the microvilli of type II alveolar cell are an indication for an active membrane transport. There are two types of lamellar bodies in the alveolar cells: the first occur in the granular pneumocyte; they are acid phosphatase negative and are certainly the source of the “surfactant”; the second occur in the alveolar macrophages; they do not exhibit a constant periodicity, they are acid phosphatase positive and are thought to represent engulfed phospholipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455269
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