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  • 11
    Electronic Resource
    Electronic Resource
    The recognition of cancer-associated fucosylated antigens in colorectal cancer by a novel monoclonal antibody, YB-2 (1994)
    Springer
    Surgery today 24 (1994), S. 382-384 
    Details
    ISSN: 1436-2813
    Keywords: fucosylated antigens ; murine monoclonal antibody ; colorectal carcer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A monoclonal antibody, YB-2, which has a novel epitope for fucosylated antigens and shows cross-reactivity with Y/Leb/H type 2 antigens, was used in an immunohisto-chemical study of human neoplastic and normal colorectal tissues. Of 64 colorectal cancers, 59 (92.2%) were stained by the YB-2 antibody; however, only 3 (12.0%) of 25 samples of normal colon mucosa and 13 (50.0%) of 26 adenomas expressed the antigens recognized by YB-2. Clinicopathologically, the YB-2-negative colon cancers were exclusively in Dukes stage A. These results indicate that the monoclonal antibody, YB-2, could detect cancer-associated antigens, and the degree of YB-2-reacted antigen expression might be correlated with the progression of colorectal carcinogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348574
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  • 12
    Electronic Resource
    Electronic Resource
    Effectiveness of Tc-99m MIBI scintigraphy in diagnosing lung cancer (1997)
    Springer
    Surgery today 27 (1997), S. 623-626 
    Details
    ISSN: 1436-2813
    Keywords: MIBI ; lung cancer ; scintigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The use of Tc-99m hexakis 2-methoxy isobutyl isonitrile (MIBI) scintigraphy for qualitative diagnosis was examined. A total of 36 lesions from 31 patients with respiratory disease (19 lesions from 19 primary lung cancer patients, 8 lesions from 3 metastatic lung cancer patients and 10 lesions from benign lung disease patients) were examined. Positive results were seen in 89.4% of the primary lung cancer lesions, 50.0% of the metastatic lung cancer lesions, and 20.0% of the benign lung disease lesions. The positive rate of 77.7% for malignant lung cancer was significantly higher (P〈0.01) than that for benign lung cancer. In particular, for lesions with a tumor diameter of 1.0 cm or more, the positive rate for malignant lung cancer lesions (95.2%) was significantly higher (P〈0.001) than that for benign lung disease lesions (25.0%). These results thus indicate Tc-99m MIBI scintigraphy to be useful for qualitative diagnosis of lung peripheral coin lesions with a diameter of 1.0 cm or more.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388218
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  • 13
    Electronic Resource
    Electronic Resource
    Increased phospholipase D activity in human breast cancer (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 280-285 
    Details
    ISSN: 1432-1335
    Keywords: Key words Phospholipase D  ;  Breast tumor  ;  Phosphatidylcholine  ;  Phosphatidylethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phospholipase D is believed to play an important role in cell proliferation and tumorigenesis. One of its major functions is to cause a sustained activation of protein kinase C through the primary production of phosphatidic acid from phosphatidylcholine by the enzyme, followed by dephosphorylation forming diacylglycerol. Protein kinase C is known to be activated or translocated in some tumors including breast tumors. In order to examine phospholipase D activity in breast tumors, surgical specimens of human breast tumors were obtained by mastectomy or wide excision, and their phospholipase D activities were assayed by determining the formation of phosphatidylethanol from phosphatidylcholine and ethanol. Phospholipase D activity was predominantly localized in the microsomal fraction of the tumor tissue and markedly stimulated by oleic acid. We observed a significant increase in phospholipase D activity in 17 out of 19 spontaneous human breast tumors as compared to adjacent histologically normal breast tissue. The mean specific activity in the tumors was 52.9 ± 41.8 (SD) pmol min−1 mg protein−1 whereas the value for the normal breast tissue was 34.0 ± 36.2 (SD) pmol min−1 mg protein−1 (P〈0.01; paired Wilcoxon's rank-sum test). The mean tumor/normal activity ratio was 2.37. Among prognostic factors, the nuclear grade, evaluated according to Schnitt et al., was found to be correlated with the activity ratio. Our results suggest a role for phospholipase D in human breast tumors. An elevation in phospholipase D activity is useful as a potential marker for malignant disease in the breast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208639
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  • 14
    Electronic Resource
    Electronic Resource
    Increased phospholipase D activity in human breast cancer (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 280-285 
    Details
    ISSN: 1432-1335
    Keywords: Phospholipase D ; Breast tumor ; Phosphatidylcholine ; Phosphatidylethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phospholipase D is believed to play an important role in cell proliferation and tumorigenesis. One of its major functions is to cause a sustained activation of protein kinase C through the primary production of phosphatidic acid from phosphatidylcholine by the enzyme, followed by dephosphorylation forming diacylglycerol. Protein kinase C is known to be activated or translocated in some tumors including breast tumors. In order to examine phospholipase D activity in breast tumors, surgical specimens of human breast tumors were obtained by mastectomy or wide excision, and their phospholipase D activities were assayed by determining the formation of phosphatidylethanol from phosphatidylcholine and ethanol. Phospholipase D activity was predominantly localized in the microsomal fraction of the tumor tissue and markedly stimulated by oleic acid. We observed a significant increase in phospholipase D activity in 17 out of 19 spontaneous human breast tumors as compared to adjacent histologically normal breast tissue. The mean specific activity in the tumors was 52.9±41.8 (SD) pmol min−1 mg protein−1 whereas the value for the normal breast tissue was 34.0±36.2 (SD) pmol min−1 mg protein−1 (P〈0.01; paired Wilcoxon's rank-sum test). The mean tumor/normal activity ratio was 2.37. Among prognostic factors, the nuclear grade, evaluated according to Schnitt et al., was found to be correlated with the activity ratio. Our results suggest a role for phospholipase D in human breast tumors. An elevation in phospholipase D activity is useful as a potential marker for malignant disease in the breast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208639
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  • 15
    Electronic Resource
    Electronic Resource
    New method of obtaining cinchophen ulcer in dogs (1977)
    Springer
    Digestive diseases and sciences 22 (1977), S. 761-768 
    Details
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cinchophen injected into the cerebral ventricle reacted with the hypothalamus somewhere in the vicinity of third ventricle, and produced a release of ACTH as indicated by a rise in peripheral plasma 11-OHCS concentration in the dog. The amount of cinchophen injected into the ventricle corresponds to about 1/600 of the dose required to produce the same effect by the intravenous route. Repeated intraventricular injections of cinchophen were effective in producing gastric erosions and ulcers at smaller doses than the intravenous one (1/100 and 1/400). Damage to the gastric wall might be due to a mechanism involving a localized portion of the hypothalamus and/or via a cerebral ventricle. Bilateral truncal vagotomy did not have any effect on chronic ulcer production. The cinchophen ulcerations were accompanied by an increase in plasma corticosteroids. The new experimental technique for producing peptic ulcer in dogs via a central neurohumoral mechanism is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01694505
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  • 16
    Electronic Resource
    Electronic Resource
    Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin (1999)
    Springer
    Molecular and cellular biochemistry 190 (1999), S. 85-90 
    Details
    ISSN: 1573-4919
    Keywords: skeletal muscle ; myosin light chain kinase ; actin-binding ; actin-myosin interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Myosin light chain kinase (MLCK) has been purified from various muscles as an enzyme to phosphorylate myosin light chains. While the regulatory role of smooth muscle MLCK is well understood, the role of skeletal muscle MLCK in the regulation of contraction has not been fully characterized. Such characterization of skeletal muscle MLCK is difficult because skeletal muscle myosin interacts with actin whether or not the myosin is phosphorylated. Taking the hint from our recent finding that smooth muscle MLCK inhibits the actin-myosin interaction by binding to actin (Kohama et al., Biochem Biophys Res Commun 184: 1204-1211, 1992), we investigated the regulatory role of the actin-binding activity of MLCK from chicken breast muscle in the actin-myosin interaction. The amount of MLCK that bound to actin increased with increases in the concentration of MLCK. However, MLCK hardly bound to myosin. The actin-binding activity of MLCK was affected when Ca2+ and calmodulin (Ca2+-CaM) were present. The effect of MLCK on the actin-myosin interaction was examined by an in vitro motility assay; the movement of actin-filaments on a myosin-coated glass surface was inhibited by increasing the concentration of MLCK. When CaM was present, the inhibition was overcome in a Ca2+-dependent manner at μM levels. The inhibition of the movement by MLCK and the recovery from the inhibition by Ca2+-CaM were not altered whether we use phosphorylated or unphosphorylated myosin for the assay, ruling out the involvement of the kinase activity of MLCK.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006925217536
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  • 17
    Electronic Resource
    Electronic Resource
    The usefulness of anti-fucosylated antigen antibody YB-2 for diagnosis of hepatocellular carcinoma (1997)
    Springer
    Glycoconjugate journal 14 (1997), S. 81-87 
    Details
    ISSN: 1573-4986
    Keywords: hepatocellular carcinoma ; glycoconjugates ; fucosylated antigens ; α-fetoprotein ; YB-2 antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Levels of fucosylated antigens in sera from patients with liver diseases were examined by a newly developed sandwich-type enzyme immuno assay with the aid of anti-fucosylated antigen antibody, YB-2 which reacts simultaneously with Y, Leb and H type 2 antigens. When the cut-off value was set arbitrarily at mean [3 SD values of normal, 30 (69.8%) of the 43 patients with HCC, 14 (53.8%) of the 26 patients with liver cirrhosis (LC) and 24 (45.3%) of the 53 patients with chronic hepatitis (CH) were found to be positive, whereas all of the 30 samples from healthy controls were negative. The levels of α-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) in HCC were not correlated with those of YB-2 antigens. The positive rates of the combination YB-2 and AFP assay and YB-2 and PIVKA-II assay in HCC were significantly higher (83.7 and 86.0%, respectively) than that of the AFP and PIVKA-II combination (65.1%) which had been reported to be the best combination up to this time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018517116368
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  • 18
    Electronic Resource
    Electronic Resource
    A clinicopathological evaluation of anti-fucosylated antigens antibody (YB-2) in colorectal carcinoma (1994)
    Springer
    Glycoconjugate journal 1 (1994), S. 31-36 
    Details
    ISSN: 1573-4986
    Keywords: anti-fucosylated antigen antibody ; colorectal cancer ; immunohistochemical diagnosis ; tumorassociated antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A newly generated monoclonal antibody, YB-2, reacts simultaneously with Y (Fucα1→2Galβ1→4[Fucα1→3]GlcNAcβ), Leb (fucα1→2Galβ1→3[Fucα1→4]GlcNAcβ) and H type 2 (Fucα1→2Galβ1→4GlcNAcβ) antigens (Jpn J Cancer Res 1993: 84; 641-8). Since these antigens have been reported to be expressed strongly in malignant colorectal tissues, we investigated the usefulness of this antibody as an immunochemical tool for diagnosis of colorectal cancer. The rate of positive staining with YB-2 antibody in colorectal carcinoma (n=101), adenoma (n=26) and normal tissues (n=25) was 95.0, 50.0 and 12.0%, respectively. The specimens with negative staining were restricted in Dukes' A patients but 75% of Dukes' C patients were strongly positive. The intensity of positive staining with YB-2 antibody was also significantly related to the clinico-pathological features such as the depth of invasion, metastasis, histological types and tumor location. Moreover, the 5-year survival in patients whose tumors were positive with YB-2 antibody was found to be significantly low. Therefore, YB-2 antibody could be useful for immunodiagnosis and, possibly, immunotherapy of colorectal carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00917466
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  • 19
    Electronic Resource
    Electronic Resource
    Peptide Transporter in the Rat Small Intestine: Ultrastructural Localization and the Effect of Starvation and Administration of Amino Acids (1999)
    Springer
    Journal of molecular histology 31 (1999), S. 169-174 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Peptide transporter-1 is a H+/peptide cotransporter responsible for the uptake of small peptides and peptide-like drugs, and is present in the absorptive epithelial cells of the villi in the small intestine (duodenum, jejunum, and ileum). It has been localized to the apical microvillous plasma membrane of the absorptive epithelial cells of the rat small intestine using the immunogold electron microscopic technique. Digital image analysis of the jejunum revealed that the transporter protein was abundant at the tip of the villus and that the amount decreased from the tip of the villus to its base. The effect of dietary administration of amino acids and starvation on the expression of PepT1 in the jejunum was examined by immunoblotting and image analysis of immunofluorescence. Starvation markedly increased the amount of peptide transporter present, whereas dietary administration of amino acids reduced it. The gradient of the transporter protein along the crypt-villus axis was maintained under either condition. These observations show that it is specific to the microvillous plasma membrane and that its expression is regulated by the nutritional condition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003515413550
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  • 20
    Electronic Resource
    Electronic Resource
    Role of the hypothalamus in the pathogenesis of cinchophen-induced gastric ulcers in dogs (1970)
    Springer
    Digestive diseases and sciences 15 (1970), S. 1083-1089 
    Details
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of hypothalamic lesions on cinchophen-induced gastric ulcers were studied in 38 male mongrel dogs. Chronic gastric ulcers were produced in 100% of the cinchophen-administered dogs without hypothalamic lesion. When cinchophen was administered to animals with anterior, posterior and lateral hypothalamic lesions, this incidence of gastric ulcers was not prevented. In dogs with lesions of the middle portion of the hypothalamus, the incidence of chronic ulcers was decreased two-thirds; in dogs with lesions of the ventromedial nuclei of the hypothalamus, goldthioglucose did not protect against the development of cinchophen ulcers. Chronic gastric ulcers were not produced in all of the dogs with lesions of the median eminence. These data suggest that daily administration of cinchophen produced gastric ulcers in the pyloric region through the mechanism of the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232873
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