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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 40 (1984), S. 303-304 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A simple and sensitive technique for staining glycoproteins on polyacrylamide gels utilizing periodic acid-Schiff (PAS) reagent is described. The novelty of this technique is an additional incubation of the PAS stained gels in an ethanolic solution of metabisulfite. The treatment results in an about 20-fold increase in the intensitiy of bands and the color is stable for at least 1 week at room temperature. Furthermore, the integrated optical density of the bands is proportional to the amount of glycoproteins. Amounts as small as 0.2 μg of fetuin can be conveniently determined. As compared to other PAS staining methods, this procedure offers a remarkably improved sensitivity for detection and quantitation of glycoproteins following their electrophoretic separation on polyacrylamide gels.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1432-1459
    Keywords: Multiple sclerosis ; Active E rosettes ; Brain homogenates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lymphocyten von Patienten mit klinisch gesicherter Multipler Sklerose reagierten in 15 von 17 Fällen je mit 4 Homogenaten von MS-Gehirnen im aktiven E-Rosetten-Test. Nur bei einem von 18 Patienten mit anderen neurologischen Leiden reagierten die Lymphocyten auf MS-Gehirnhomogenate. Die genaue Natur des stimulierenden Agens im MS-Gehirn ist noch unbekannt.
    Notes: Summary Lymphocytes from 15 of 17 patients with clinically well defined multiple sclerosis (MS) responded to all four MS brain homogenates as assayed by active E rosette test. Lymphocytes from only one neurological patient, out of 18 tested, responded to MS brain homogenates. The nature of the stimulating agent from MS brains remains to be determined.
    Type of Medium: Electronic Resource
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