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  • 11
    Digitale Medien
    Digitale Medien
    The gene family encoding the fucoxanthin chlorophyll proteins from the brown alga Macrocystis pyrifera (1995)
    Springer
    Molecular genetics and genomics 246 (1995), S. 455-464 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Blue light ; Chromophyta ; Fucoxanthin ; Macrocystis ; Phaeophyta ; Signal sequence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Six members of a multigene family encoding polypeptide constituents of the fucoxanthin, chlorophyll a/c protein complex from female gametophytes of the brown alga Macrocystis pyrifera have been cloned and characterized. The deduced amino acid sequences are very similar to those of fucoxanthin chlorophyll binding proteins (Fcp) from the diatom Phaeodactylum tricornutum and exhibit limited homology to chlorophyll a/b binding (Cab) polypeptides from higher plants. The primary translation products from the M. pyrifera fcp genes are synthesized as higher molecular weight precursors that are processed prior to their assembly into the Fcp complex. The presumed N-terminal 40-amino acid presequence of the Fcp precursor polypeptide has features resembling that of a signal sequence. This presequence may be required for the protein to transverse the endoplasmic reticulum that surrounds the plastid in brown algae. A subsequent targeting step would be required for the protein to cross the double membrane of the plastid envelope. M. pyrifera fcp transcripts are of two sizes, 1.2 and 1.6 kb. The size difference is accounted for by the length of the 3′ untranslated region, which can be up to 1000 bases. Transcript abundance's of members of the fcp gene family are dependent on light quantity, light quality, or both. Transcript levels of one gene increased approximately five- to tenfold in thalli grown in low intensity relative to high intensity white or blue light. Transcripts from this gene also significantly increase in red light relative to blue light at equivalent light intensities.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00290449
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  • 12
    Digitale Medien
    Digitale Medien
    Isolation and Characterization of an Actin Promoter from the Red Abalone (Haliotis rufescens) (1999)
    Springer
    Marine biotechnology 1 (1999), S. 269-278 
    Details
    ISSN: 1436-2236
    Schlagwort(e): Key words: 5′ untranslated region, gene transfer, intron, mollusk, phylogeny, transfection.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract: We have isolated and characterized the 5′-flanking and part of the coding region of an actin gene from the red abalone Haliotis rufescens. There is high sequence homology between the abalone actin coding region and actins from other species. The sequence of this abalone actin is more closely related to vertebrate cytoplasmic actins than to muscle actins. RNase protection assays located the position of the transcription start point 66 bp upstream of the initiation codon. Promoter prediction by neural network located a TATA box 30 bp upstream of the transcription start point. A search with the SIGNAL SCAN program identified several potential transcription factor binding sites in the abalone sequence. These sites include sequences highly conserved in other actin promoters, like several putative CAAT and E boxes and a modified CArG box. Transfection assays with a construct containing the 5′ flanking region of the abalone actin coupled to a luciferase reporter gene showed that the promoter is functional in mammalian and fish cell lines, as well as in abalone gonad tissue. Expression vectors constructed with the abalone actin promoter will be useful for gene transfer studies into abalone and other mollusks.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00011776
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  • 13
    Digitale Medien
    Digitale Medien
    Glucosephosphate isomerase (GPI) of the teleostFundulus heteroclitus (linnaeus): Isozymes, allozymes and their physiological roles (1980)
    Springer
    Journal of comparative physiology 138 (1980), S. 49-57 
    Details
    ISSN: 1432-136X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In order to evaluate the role of glucose-phosphate isomerase (GPI) inFundulus heteroclitus, the isozymes and allozymes were purified and some of their physical and kinetic properties determined. Isozymes were purified from both liver (GPI-B) and muscle (GPI-A) tissue (Tables 1, 2). Gel filtration of the native enzyme and SDS-polyacrylamide gel electrophoresis indicated that all forms are dimers of approximately 110,000 Daltons (Figs. 4, 5). Although thermal stability studies revealed no differences between the allozymes, the isozymes were clearly different (Figs. 6, 7). Kinetic analysis showed further differences between isozymes inK m for substrate andK I for 6-phosphogluconate (Figs. 8, 9; Table 3). No significant differences were found between the allozymes of the B-locus under the conditions employed in this study. Based on the tissue specificities and the functional differences between isozymes, we propose a possible regulatory role for GPI-B inF. heteroclitus. The sensitivity of this isozyme to 6-phosphogluconate inhibition may allow GPI-B to act as a regulatory enzyme in the partitioning of carbon flow between glycolysis and the hexose monophosphate shunt.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688735
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  • 14
    Digitale Medien
    Digitale Medien
    pH Dependence of 2,3-diphosphoglycerate binding to human hemoglobin Ao at 21.5°C (1986)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 1 (1986), S. 164-175 
    Details
    ISSN: 0887-3585
    Schlagwort(e): DPG ; organophosphates ; ligand interactions ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Rate equilibrium dialysis was used to measure the binding of 2,3-diphosphoglycerate (DPG) to human oxy- and deoxyhemoglobin AO over the range pH 5-9, at 21.5°C. This approach yielded an accurate, precise, and self-consistent set of model-independent association constants. These data were successfully fitted to a thermodynamic model which is fuctionally similar to a Hill equation. The isotherms generated by this fitting procedure appear to intersect at low pH and converge at high pH. This apparent convergence at high pH is consistent with results obtained by oxygen equilibria studies performed under conditions of saturating DPG. These calculated isotherms were used to determine the enhancement of the Bohr effect as a function of pH. These results are consistent with data obtained by pH stat measurements by other investigators.This paper presents the first in a series of studies that will provide a systematic characterization of the interaction between hemoglobin and DPG.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prot.340010208
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  • 15
    Digitale Medien
    Digitale Medien
    Gene transfer, expression and inheritance of PRSV-rainbow trout-GH cDNA in the common carp, Cyprinus carpio (Linnaeus) (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 25 (1990), S. 3-13 
    Details
    ISSN: 1040-452X
    Schlagwort(e): Growth hormone ; Transgenic fish ; Exogenous DNA integration ; Offspring ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: A recombinant plasmid containing the Rous sarcoma virus-long terminal repeat (RSV-LTR) promoter linked to rainbow trout (Salmo gairdneri) growth hormone (GH) cDNA was microinjected into fertilized carp eggs. Genomic DNA extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and Southern blot hybridization, using the RSV-LTR and/or the GH cDNA sequences as probes. Out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain pRSV-rtGH-cDNA sequence in the genomic DNA. Expression of the trout GH polypeptide was detected by immunobinding assay in the red blood cells of nine transgenic fish tested. The level of expression, however, varied among the transgenics and could not be correlated with exogenous DNA copy number. Although there was considerable variation in the sizes of the transgenic fish, those microinjected during the one-cell stage were (P 〈 0.05) 22% larger, on the average, than their sibling controls. A randomly selected fraction of the progeny derived from crosses between transgenic males and non-transgenic females inherited the foreign DNA. These transgenic progeny grew faster (P 〈 0.05) than their non-transgenic siblings.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1080250103
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