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  • 11
    Electronic Resource
    Electronic Resource
    The occurrence of estrogens in relation to reproductive processes in flowering plants (1991)
    Springer
    Sexual plant reproduction 4 (1991), S. 193-196 
    Details
    ISSN: 1432-2145
    Keywords: Estradiol ; Estrogens ; Pollination ; Radioimmunoassay ; Lilium ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Estrogens, a group of steroidal sex hormones, were determined in pollen, styles or whole pistils using a highly sensitive and specific radioimmunoassay (RIA) and found to be present at levels between 10−11 and 10−10 g/g FW. In the style of Liliun davidii Duch., the levels of total estrogen decreased after self-pollination of the open flower. After bud pollination, which partly overcame incompatibility, the levels of both estradiol and total estrogen increased. The levels of estradiol and total estrogen in the pistils of Brassica pekinensis Rupr. increased after compatible pollination during the first 3 days following pollination and decreased thereafter when the data were expressed on a FW basis. These results suggest that estrogens are to some extent associated with the reproductive processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190004
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  • 12
    Electronic Resource
    Electronic Resource
    Book reviews (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 447-448 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273753
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  • 13
    Electronic Resource
    Electronic Resource
    Covalently bound wall proteins of pollen grains and pollen tubes grown in vitro and in styles after self- and cross-pollination in Lilium longiflorum (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 263-267 
    Details
    ISSN: 1432-2242
    Keywords: Covalently bound wall proteins ; Pollen ; Pollen tubes ; Tube growth ; Incompatibility ; Lily ; Lilium longiforum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method was worked out using trifluoromethanesulfonic acid (TFMS) as a reagent to split the covalently bound proteins, which are NaCl insoluble, from pollen tube walls of Lilium longiflorum, leaving the peptide bonds essentially intact. After electrophoretic separation, comparisons were made among these proteins from pollen grains and pollen tubes grown in vitro and in styles after self- and cross-pollination. It was found that a) the patterns of covalently bound wall proteins were different between tubes grown in vitro and in vivo; b) fewer bands were found in covalently bound wall proteins than that in noncovalently bound proteins; c) the bands remained almost the same no matter whether the tubes had been cross pollinated or self pollinated, indicating that while the noncovalently bound proteins were involved in incompatibility as shown in the previous paper, the covalently bound proteins may only serve as a structural component, having little to do with incompatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252065
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  • 14
    Electronic Resource
    Electronic Resource
    Detection and immunolocalization of glycoproteins of the plasma membrane of maize sperm cells (1997)
    Springer
    Protoplasma 198 (1997), S. 125-129 
    Details
    ISSN: 1615-6102
    Keywords: Glycoproteins ; Peroxidase-conjugated concanavalin A ; Plasma membrane ; Sperm cell ; Zea mays ; Indirect immune localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary After purifying plasma membranes from isolated maize sperm cells by aqueous polymer two-phase partition, peripheral and integral proteins were solubilized from the plasma membrane with Triton X-114 and separated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Silver staining revealed 10 bands (19–68 kDa) of peripheral membrane proteins and about 40 bands (12–120 kDa) of integral proteins. Peroxidase-conjugated Con A was used to detect the surface glycopeptides. It was found that Con A particularly stained 8 peripheral polypeptide bands, including 68, 66, 55, 51,48, 44, 36, and 32 kDa, and 6 integral polypeptide bands, 68, 51, 48, 44, 38, and 34 kDa. These bands differed from those of somatic samples. Staining specificity was demonstrated by the control in the presence of competing inhibitory sugar. The above result indicates the existence of mannosyl and glucosyl residues in the surface glycoproteins of maize sperm cells. The prominent peripheral 68 kDa polypeptide was further separated into 4 spots by isoelectric focusing and sodium dodecyl sulfate two-dimensional (IEF-SDS 2-D) electrophoresis, showing pI values from 5.5 to 5.8. Three prominent glycopeptides (68, 48, and 32 kDa) were localized on the plasma membrane of maize sperm cells via the fluorescein isothiocyanate (FITC) technique. About 25% of sperm cells showed an intense positive reaction in each immunological labelling. The results agree with our previous labelling of the surface of isolated viable maize sperm cells with Con A-FITC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287560
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    Paper (German National Licenses)
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