ISSN:
1573-6903
Keywords:
Major vault protein
;
electric ray cholinergic nerve terminal
;
protein kinase C
;
casein kinase II
;
protein tyrosine kinase
;
ribonucleoprotein particle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The major vault protein is the predominant member of a large cytosolic ribonucleoprotein particle, named vaults. Vaults are abundant in nerve terminals of the electric organ of Torpedo marmorata.Negative staining of isolated vaults reveals particle dimensions of 45 × 65 nm in size. Comparison of the major vault protein (MVP 100) from the two electric ray species Torpedo marmorataand Discopyge ommatareveals few microheterogeneities in amino acid sequence. Potential phosphorylation sites for various protein kinases are highly conserved. Phosphorylation studies demonstrate that the major vault protein of Torpedois a substrate of various protein kinases. MVP100 is phosphorylated by protein tyrosine kinase in vivo and protein kinase C and casein kinase II in vitro. Inhibitors and activators of protein kinases specifically modulate the phosphorylation of MVP 100.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022445302710
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