ISSN:
1432-1912
Keywords:
Dopamine autoreceptors
;
Pergolide
;
Apomorphine
;
Dopamine antagonists
;
Tyrosine hydroxylase
;
Rat striatal miniprisms
;
Rat limbic miniprisms
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A method for the assay of tyrosine hydroxylase activity in rat striatal and limbic (nucleus accumbens + olfactory tubercle) brain miniprisms is described. The dopamine agonists apomorphine (1 μmol/l) and pergolide (0.01–100 μmol/l) inhibited the tyrosine hydroxylase activity in both regions. The inhibition produced by 1 μmol/l pergolide was antagonised partially in striatal miniprisms and completely in limbic miniprisms by 1 μmol/l haloperidol. The dopamine D2-selective antagonist raclopride, at concentrations up to 300 nmol/l, did not antagonise the inhibition produced by pergolide in striatal miniprisms, but appeared partially to antagonise the inhibition in limbic miniprisms. It is concluded that whilst pergolide potently inhibits tyrosine hydroxylase activity in striatal and limbic miniprisms, the inhibition is of doubtful value as a predictive model of dopamine autoreceptor function in striatal miniprisms, but may be useful when limbic miniprisms are used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00500008
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