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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 38 (1993), S. 599-605 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid productivities from chemostat cultures of an isolate of Isochrysis galbana have been studied. The productivities reached in the interval of dilution rates between 0.0295 h−1 and 0.0355 h−1 were 1.5mg·1−1·h−1 for lipids, 300 μg·1−1·h−1 for EPA and 130μg1·1−1·h−1 for DHA. Furthermore, light attenuation by mutual shading, and agitation speed influences on growth and fatty acid composition were analysed. A model relating steady-state dilution rates to internal average light intensity has been proposed, the parameter values of which obtained by non-linear regression were: maximum specific growth rate (μmax)=0.0426 h−1; the affinity of cells to light (Ik) = 10.92 W·m−2; the exponent (n) = 5.13; regression coefficient (r 2)=0.9999.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5176
    Keywords: chemostat culture ; Isochrysis galbana ; fatty acids ; solar cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Outdoor chemostat cultures of the marine microalgaIsochrysis galbana at constant dilution rate (0.034 h−1 ) have been carried out under different weather conditions. Steady-state biomass concentrations were 1.61±0.03 kg m−3 in May and 0.95±0.04 kg m−3 in July, resulting in biomass output rates of 0.54 kg m−3 d−1 and 0.32 kg m−3 d−1 in May and July, respectively. Two patterns of daily variation with the solar cycle were observed in the fatty acid content. Saturated and mono-unsaturated fatty acids (16:0 and 16:1n7) show significant variation with the solar cycle, associated with short-term changes in environmental factors. Palmitic and palmitoleic acids are generated during daylight and consumed during the dark period. However, polyunsaturated fatty acids do not show a significant response to the solar cycle and their changes are associated with long-term variation in environmental factors. The maximum EPA productivity was obtained in May, 14.1 g m−3 d−1, which is close to that found in the literature for indoor continuous cultures. Nonetheless, the outdoor EPA content (up to 2.61 % d.wt) was lower than the indoor EPA content from a previous study (5% d.wt).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The simultaneous effect of temperature and photon flux density on microalga Chlorella sp. growth was analysed by response surface methodology of two consecutive full factorial designs. Maximum specific growth rate was 0.128 h-1 at 35°C and 2400 μmol photon m-2s-1. A photoinhibition effect was observed at high photon flux densities with temperatures far below the optimum. Temperature was the main factor affecting specific growth rate.
    Type of Medium: Electronic Resource
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