ISSN:
1573-5060
Keywords:
genetic stability
;
Solanum tuberosum
;
rDNA
;
slow growth
;
cryopreservation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Ribosomal gene (rDNA) probes have been used to assess genomic changes in plants of (1) S. tuberosum cv Desiree subjected to slow growth and (2) S. tuberosum cv Golden Wonder recovered from cryopreservation. Restriction fragment length polymorphisms (RFLPs) were detected in two out of 16 DNA samples extracted from plants derived from slow growth, control plants did not show RFLP differences. Plants recovered from cryopreserved shoot-tips of Golden Wonder were unchanged in their ribosomal gene RFLP profile compared to the untreated controls. The use of rDNA probes as tools to assess stability, and the possible detrimental effects of slow growth, somaclonal variation, cryoprotectants, and freezing injury are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00025226
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