ISSN:
1432-203X
Keywords:
Hybrid rice
;
RAPD
;
PCR
;
Oryza sativa
;
DNA fingerprinting
;
Molecular markers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary DNA from three families of rice plants selected in Northern China (each comprising the male sterile, the restorer, the hybrid F1 and the maintainer lines) has been extracted and amplified by PCR with different random DNA primers (RAPD analysis). Then, DNA has been analysed by agarose gel electrophoresis and DNA bands scored as present or absent. The generated matrices are reproducible and amenable for identification of each single plant line. Thus, RAPD fingerprinting of the inbred parental lines and of the resulting hybrid is proposed as a convenient tool for the identification, protection and parentage determination of plant hybrids. Furthermore, by offering a molecular tool to verify the degree of dissimilarity between the parental lines, the RAPD analysis may also be used to search for new parental combinations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233772
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