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  • 1
    Electronic Resource
    Electronic Resource
    Parallel stimulation of mitochondrial H+ (Mg2+-ATPase and rate of germ tube outgrowth from conidia of Neurospora crassa (1983)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 20 (1983), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Mitochondrial H+ (Mg2+)-ATPase (EC 3.6.1.3) activity of Neurospora crassa has been studied during conidial germination in the presence and absence of 2,4-dinitrophenol and dicoumarol. The activity was optimal at pH 8.5–9.0 and was inhibited (80–90%) by oligomycin, N,N'-dicyclo-hexylcarbodiimide and azide.The enzyme activity already present in mitochondria isolated from dry- and wet-harvested conidia did not change significantly during the 2.5 h pregermination at 25°C (20% outgrowth), but increased sharply up to 7.5 h corresponding to the end of germination period and remained steady until the end of log phase (15 h).In uncoupler-treated conidia, the stimulation of germ tube outgrowth at 2.5 h was found to parallel the activation (40%) of mitochondrial ATPase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1983.tb00126.x
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  • 2
    Electronic Resource
    Electronic Resource
    Heat-induced changes in respiratory pathways and mitochondrial structure during microcycle conidiation of Neurospora crassa (1980)
    Springer
    Archives of microbiology 125 (1980), S. 53-58 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Microcycle conidiation ; Heat-treatment ; Cyanide-sensitive and insensitive respirations ; Mitochondrial structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in both respiratory pathways and mitochondrial structure of Neurospora crassa occurred under conditions of microcycle conidiation. Upon heat-treatment at 46°C, conidia developed a highly cyanide-insensitive, hydroxamate-sensitive respiration associated with morphological alterations in mitochondrial membranes; such changes were time-dependent. When heat-treated conidia were shifted down to 25°C, the alternate, hydroxamate-sensitive respiration decreased significantly, paralleling the recovery of well-cristated mitochondria with an electron-dense matrix in the germ tubes. The decrease in hydroxamate-sensitivity was associated with two periods of increase in cyanide sensitivity corresponding to the events of germination and precocious proconidial budding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403197
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of loss and recovery of cytochrome oxidase activity during and after heat induction of microcycle conidiation inNeurospora crassa (1983)
    Springer
    Protoplasma 116 (1983), S. 149-154 
    Details
    ISSN: 1615-6102
    Keywords: Cytochrome oxidase ; Cytochemistry ; Ultrastructure ; Neurospora ; Heat treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytochrome oxidase (EC 1.9.3.1) activity of conidia of wild-typeNeurospora crassa was detected cytochemically at the ultrastructural level by 3,′-diaminobenzidine (DAB) reaction during heat treatment at 46 °C and after shift down to 25 °C. At 46 °C the decrease in the enzyme activity was shown to be time-dependent (0–10 hours) in the progressively overswelling conidia. Gradual recovery of the DAB reaction occurred in mitochondria of conidia outgrowing conidiogenous germ tubes (microcycle conidiation) when shifted down to 25 °C. Inactivation of cytochrome oxidase was efficiently prevented during heat treatment in the presence of Tween 20. Such conidia no longer overswelled but still required shift down to 25 °C to produce germ tubes with delayed conidiogenous ability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279832
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    Paper (German National Licenses)
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