ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Optimal agitation and aeration conditions [assuring O2 transfer rates (OTR) from 12 to 179 mmol L-1 h-1] were determined for pectin lyase (PL) synthesis of an Aspergillus niger strain. Components of the pectolytic enzyme complex were also investigated in order to determine whether their O2 demand is identical with or different from that of pectin lyase. Should the latter be the case, a possibility would be given to produce enzyme complexes of different agitation and aeration conditions. According to our results, mycelium yield of Aspergillus niger attained a maximum at an OTR of 100 mmol L-1 h-1. The yields of the various pectolytic enzymes reached maxima at different OTRs. Pectin lyase production was the highest (0.555 µmol min-1 mL-1) at an OTR of 60 mmol L-1 h-1. Endopolygalacturonase (PG) production showed a maximum at the OTR of 49 mmol L-1 h-1 with a second peak at 100-135 mmol O2 L-1 h-1. Pectin esterase (PE) synthesis showed a maximum at on OTR of 12-14 mmol L-1 h-1, while both apple juice clarifying and macerating activities gave two maxima at 14 and 60 mmol L-1 h-1 due to the optima of PE and endo-PG. Macerating activity showed a high value at OTR optimal for PL production as well.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260231007
Permalink
