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Phylogenetic Distribution of Peptides Related to Chromogranins A and B (1988)

Rieker, Susanne ; Fischer-Colbrie, Reiner ; Eiden, Lee ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 50 (1988), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: The presence of chromogranin-related peptides in a wide range of species was investigated by one- and two-dimensional electrophoresis followed by immunoblotting. Antisera against bovine chromogranins A and B and the peptide WE-14 (chromogranin A316–329) were used. Chromogranins were identified by their heat stability, by their electrophoretic behavior, and by immunological cross-reaction with antisera. In all species investigated ranging from mammals to birds, amphibians, fish, and arthropods, chromogranin A- and B-like proteins could be demonstrated. For all species, there was an immunological cross-reaction with antisera against bovine chromogranins. The molecular sizes and isoelectric points of the chromogranins were similar in all species. The antiserum against WE-14 cross-reacted with pig, rat, and chicken chromogranins. It is concluded that the chromogranins A and B have a widespread phylogenetic distribution with a significant conservation of molecular size, isoelectric points, and immunological epitopes. This is consistent with the concept that these peptides have a specific function.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb10574.x

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Direct Observation of 103Rh-Chemical Shifts in Mono- and Dinuclear Olefin ComplexesTransition Metal NMR. Spectroscopy, Part III; Part II see [1]. (1982)

Maurer, Ellen ; Rieker, Susanne ; Schollbach, Michael ; [et al.]

New York, NY : Wiley-Blackwell

Helvetica Chimica Acta 65 (1982), S. 26-45

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ISSN: 0018-019X

Keywords: Chemistry ; Organic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The 103Rh-resonance has been investigated at 2.8 MHz in mono- and dinuclear olefin complexes by steady-state NMR.-pulse techniques. For dirhodium complexes with scalar Rh, Rh-coupling, a selective rf-irradiation was applied to improve sensitivity. Within the same measuring time the signal/noise ratio is improved by the factor (T2/T2*)1/2 by this steady-state method in comparison with the usual free-induction technique. (T1/T2)-ratios, as obtained from the dependence of signal in tensity on the pulse-flip angle are discussed in terms of relaxation mechanisms and chemical exchange phenomena. From (T1/T2)- and (T1 + T2)-experiments, individual T1 and T2 values have been determined for C5H5RhC8H12 and Rh(acac)3.The 103Rh-chemical shifts of 39 olefin complexes are reported. Shielding depends upon the (formal) oxidation state of Rh whereby resonances of pure olefin complexes appear at the low-frequency end of the δ-scale, which extends over 10000 ppm. For cyclic 1,3-diene ligands Rh-shielding increases with decreasing ring size and decreases with further conjugation of the diene systems.In the dirhodium complexes, Rh, Rh spin-coupling constants are 〈 10 Hz. Rh, C-coupling constants in (diene)RhCp-complexes increase, for the terminal diene C-at om, with increasing ring size of the 1,3-diene, 1J(Rh, C) = 11.7 to 17.4 Hz.

Additional Material: 7 Ill.