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  • 1
    ISSN: 1432-0789
    Keywords: Key words Land use ; Mineralization ; Nitrogen availability ; Oxisol ; Nitrogen management index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effect of land use on the availability of nitrogen (N) was studied by separating total N into a labile and a stable fraction by oxidation and extraction of labile N with KMnO4. The nitrogen management index (NMI) was calculated following the method of Blair et al. (1995) for the carbon management index. In all systems, labile N released by KMnO4 was a better indicator of N availability than total and stable N. The NMI was a good indicator of N availability but gave no information about the total amount of N. In land use system analysis, total N and labile N can be used together as a simple and rapid way to evaluate the N status of the soil. Legume-based pastures specifically increased the amount of labile N. Although soybeans had a dominant role in the continuous cropping systems studied, the total N contents of these systems were lower than those of the natural Cerrado. The availability of N under legume-based pastures and legume-based pasture-crop rotations was higher than under the natural Cerrado and the continuous cropping systems.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A proinsulin analog constructed by site-directed mutagenesis was expressed as a fusion protein that formed inclusion bodies inside the cells. It was purified from the isolated inclusion bodies and proinsulin was obtained by trifluoro-acetic acid, dimethyl sulfoxide and hydrochloric acid cleavage. The released proinsulin analog was confirmed by its molecular weight as determined by SDS-PAGE.
    Type of Medium: Electronic Resource
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