ISSN:
1432-072X
Keywords:
Archaebacteria
;
Methanogens
;
Methanocorpusculum parvum
;
Taxonomy
;
16 S rRNA
;
Serological probes
;
Physiology
;
Tungsten
;
Cofactors
;
Cytochrome
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A new mesophilic, monotrichously flagellated methane-producing coccus of ≦1μm in diameter was isolated from an anaerobic sour whey digester, originally inoculated with sewage sludge. Growth and methane production were observed with H2/CO2, formate and — less effectively — with 2-propanol/CO2. The isolate grew at temperatures between 15° C and 45° C with the optimum at around 37° C. Acetate, yeast extract and tungstate were required in the medium. Clarified rumen fluid stimulated growth. The DNA of the new methanogen has a G+C content of 48.5 mol%. Comparative 16 S rRNA oligonucleotide cataloguing allows to define the new isolate as a member of a new genus of the order Methanomicrobiales. Further evidence for this is provided by the antigenic crossreactivity with anti-S probes and by metabolic features. Because of its small size the new methanogen is named Methanocorpusculum parvum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00492898
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