ISSN:
1432-072X
Keywords:
β-Glucosidase
;
Lagenidium
;
Mosquitoes
;
Oomycetes
;
Parasitism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Lagenidium giganteum Couch, a watermold parasitic on mosquito larvae possesses an intracellular β-d-glucosidase (β-d-glucoside glucohydrolase, E. C. 3.2.1.21). The enzyme was purified 132 fold and, with the substrate p-nitrophenyl β-d-glucoside, was shown to have the following properties: The pH of optimum enzyme stability and activity lay between 5.5 and 6.0, and the enzyme was inactivated at temperatures above 50°C. The K m was 4.6×10-5 M and the Arrhenius activation energy was 8.35 Kcal·mole-1. Elution from Sephadex G-200 gave an approximate molecular weight of 120000. The enzyme was inhibited by Pb2+, Ag2+ and Hg2+, by glucose and by p-chloromercuribenzoate. The latter inhibition was overcome cy cysteine. Chromatographic studies demonstrated transferase as well as hydrolase properties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00455950
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