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  • 1
    Electronic Resource
    Electronic Resource
    Class IIa bacteriocins: biosynthesis, structure and activity (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 24 (2000), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In the last decade, a variety of ribosomally synthesized antimicrobial peptides or bacteriocins produced by lactic acid bacteria have been identified and characterized. As a result of these studies, insight has been gained into fundamental aspects of biology and biochemistry such as producer self protection, membrane–protein interactions, and protein modification and secretion. Moreover, it has become evident that these peptides may be developed into useful antimicrobial additives. Class IIa bacteriocins can be considered as the major subgroup of bacteriocins from lactic acid bacteria, not only because of their large number, but also because of their activities and potential applications. They have first attracted particular attention as listericidal compounds and are now believed to be the next in line if more bacteriocins are to be approved in the future. The present review attempts to provide an insight into general knowledge available for class IIa bacteriocins and discusses common features and recent findings concerning these substances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.2000.tb00534.x
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning of two (R)-specific enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 184 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two Pseudomonas aeruginosa genes, termed phaJ1Pa and phaJ2Pa, homologous to the Aeromonas caviae (R)-specific enoyl-CoA hydratase gene (phaJAc) were cloned using a PCR technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (PHA) synthesis from β-oxidation cycle. Two expression plasmids for phaJ1Pa and phaJ2Pa were constructed and introduced into Escherichia coli DH5α strain. The recombinants harboring phaJ1Pa or phaJ2Pa showed high (R)-specific enoyl-CoA hydratase activity with different substrate specificities, that is, specific for short chain-length enoyl-CoA or medium chain-length enoyl-CoA, respectively. In addition, co-expression of these two hydratase genes with PHA synthase gene in E. coli LS5218 resulted in the accumulation of PHA up to 14–29 wt% of cell dry weight from dodecanoate as a sole carbon source. It has been suggested that phaJ1Pa and phaJ2Pa products have the monomer-supplying ability for PHA synthesis from β-oxidation cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09013.x
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  • 3
    Electronic Resource
    Electronic Resource
    Production of acetone, butanol and ethanol from palm oil waste by Clostridium saccharoperbutylacetonicum N1-4 (1995)
    Springer
    Biotechnology letters 17 (1995), S. 649-654 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A study of the feasibility of utilizing palm oil waste as a substrate for acetonebutanol-ethanol fermentation by Clostridium saccharoperbutylacetonicum N1-4 and, hence, to reduce the biochemical oxygen demand (BOD) of waste disposal was carried out. Among the two types of palm oil waste tested, separator sludge was the better substrate and it was able to support production of solvents by C. saccharoperbutylacetonicum N1-4 without a need for any mineral supplements. Enzymatic hydrolysis catalyzed by cellulase prior to fermentation was found to increase the yield of butanol by 75% (from 2.47g/l to 4.37g/l) and decreased the BOD by 66% (from 26,500 ppm to 8,900 ppm).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129394
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  • 4
    Electronic Resource
    Electronic Resource
    L-lactate production from xylose employingLactococcus lactis IO-1 (1992)
    Springer
    Biotechnology letters 14 (1992), S. 599-604 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The growth, substrate utilisation and L-lactate production ofLactococcus lactis IO-1 were examined on xylose, and glucose and xylose media. The yield of lactate on xylose was 0.47 g lactate/g xylose at an initial xylose concentration of 51.2 g/l and the μmax was 0.72 h−1. Xylose cultures were more susceptible to lactate inhibition than were glucose cultures but showed similar kinetic behaviour. The organism was capable of complete sugar utilisation when grown on a mixture of 20 g/l xylose and 20 g/l glucose and synthesised 0.66 g lactate/g sugar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01023948
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  • 5
    Electronic Resource
    Electronic Resource
    A new method for the accurate and rapid determination of the concentrations of intracellular metabolites in cells during fermentation (1995)
    Springer
    Biotechnology techniques 9 (1995), S. 409-412 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A method based on density gradient centrifugation for the accurate and rapid determination of concentrations of intracellular metabolites was developed. The new method was applied to determination of intracellular levels of lactate during lactate fermentation and of intracellular levels of glutamate during glutamate fermentation. The method gave satisfactory results, showing good reproducibility and reliability with a probability of 95%. This method will allow basic information to be obtained about the transport of metabolites from within cells to the culture broth and about dynamic changes in metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160827
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  • 6
    Electronic Resource
    Electronic Resource
    Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus (1993)
    Springer
    Biotechnology letters 15 (1993), S. 1217-1222 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130300
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  • 7
    Electronic Resource
    Electronic Resource
    Continuous culture systems involving Zymomonas mobilisand a hollow fiberfilter module (1997)
    Springer
    Biotechnology techniques 11 (1997), S. 537-541 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Culture systems involving the use of one or two fermenters in series, with cell recycle employing hollow fiber filter modules to achieve high cell densities, were developed. Dilution rates of about 0.6 h -1 and ethanol productivity values of about 25 g/l h -1 , with residual glucose at 0.9 g/l, could be achieved using the system employing one fermenter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018478419923
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  • 8
    Electronic Resource
    Electronic Resource
    Optimization of substrate feed for continuous production of lactic acid by Lactococcus lactis IO-1 (1996)
    Springer
    Biotechnology letters 18 (1996), S. 1113-1118 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Optimization of substrate feed for continuous production of lactic acid by the homofermentative bacterium, Lactococcus lactis IO-1, in glucose medium was investigated. A pH-dependent feed with two pH set-points, a lower set-point for neutralization with alkali and an upper set-point for substrate feed, proved better than continuous substrate feed with one pH set-point for neutralization with alkali only. Built-in electrodialysis with a cell-recycling system was tested and high cell density was achieved as a result of the use of enriched medium. However, specific lactate productivity in this system was not satisfactorily high. pH-dependent feed was combined with turbidity control and a cell recycling. With this system, we achieved high specific lactate productivity of 2 g (g-cell)-1 h-1 at a dilution rate of 0.5 h-1, a dry cell weight of 5 g l -1, a level of lactate in the broth of 20 g l -1, and a concentration of glucose in the spent medium of about 5 gl -1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128576
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  • 9
    Electronic Resource
    Electronic Resource
    The kinetics of end-product inhibition of l-lactate production from xylose and glucose by Lactococcus lactis IO-1 (1993)
    Springer
    Biotechnology letters 15 (1993), S. 489-494 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The relative contributions of lactate inhibition and the generation of sterile (undividing) cells to the low xylose utilisation rate of Lactococcus lactis IO-1 was investigated. The lactate inhibition constant of xylose grown cells was shown to be 9.3 times more than that of glucose grown cells. However, the sterile cell production rate and LDH inactivation rate of the xylose cultures were at least 10 times less than the glucose cultures. Thus, it is suggested that the slower substrate consumption rate in xylose medium is caused mainly by the large inhibition constant for the end product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129324
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  • 10
    Electronic Resource
    Electronic Resource
    Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method (2000)
    Springer
    Biotechnology letters 22 (2000), S. 1067-1069 
    Details
    ISSN: 1573-6776
    Keywords: Alcaligenes eutrophus ; biodegradable polyester ; pH-stat substrate feeding ; propionic acid ; P(3HB-co-3HV)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The feeding of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] by Alcaligenes eutrophus ATCC17697 was optimized using a fed-batch culture system. The concentration of propionic acid was maintained at 3 g l−1 as growth was inhibited by propionic acid in the broth. A pH-stat substrate feeding system was used in which propionic acid was fed automatically to maintain a pH of the culture broth at 7.0. By feeding a substrate solution containing 20% (w/v) propionic acid, 4.9% (w/v) ammonia water [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10] in cell growth phase, the concentration of propionic acid in the broth was maintained at 3 g l−1 giving a specific growth rate of 0.4 h−1. To promote P(3HB-co-3HV) production, two stage fed-batch culture which consisted of the stage for the cell growth and the stage for the P(3HB-co-3HV) accumulation was carried out. When the substrate solution whose C/N molar ratio was 50 was fed in P(3HB-co-3HV) accumulation phase, the cell concentration and the P(3HB-co-3HV) content in the cells reached 64 g l−1 and 58% (w/w) in 55.5 h, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005650132371
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