ISSN:
0006-3592
Keywords:
cell cycle dependency
;
recombinant yeast
;
rice α-amylase
;
synchronous culture
;
arrested culture
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The cell cycle dependency of rice α-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using α-mating factor and a cdc28 mutant, indicated that the rice α-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using α-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. The intracellular content of rice α-amylase was also measured during the cell cycle. Like the specific production rate, the intracellular content was found to fluctuate throughout the cell cycle, and to reach a maximum during M phase. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 262-271, 1997.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
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