Electronic Resource
Springer
Cellular and molecular life sciences
44 (1988), S. 348-351
ISSN:
1420-9071
Keywords:
L. lactis
;
protoplasts
;
protoplast regeneration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary More than 99% ofL. lactis cells have been converted to protoplasts upon digestion of cell walls with mutanolysin (N-(acetyl)muramidase). Functional protoplasts were obtained even with the lowest level of the enzyme that was used (0.1 U·ml−1 of the cell suspension) and after incubation at 37°C for 2 min. The regeneration of the polymerized cell wall appears to be induced by a cell homogenate of the same organism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01961279
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