ISSN:
1365-2559
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
To evaluate a recently developed technique allowing the removal and processing for histology of thick tissue fragments, called microbiopsies, from non-cervical cytology specimens.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and resultsForty-five non-cervical smears from malignant tumours which contained microbiopsies were selected and processed. Sufficient sections could be cut in most cases for haematoxylin and eosin and an extensive panel of immunostaining. Seventy-one per cent of histological slides from the microbiopsies were representative of the tumour and confirmed the diagnosis. In 29% of the cases they were too small, contained non-representative tissue or showed extensive necrosis. Surprisingly, immunostaining results were at least the same and often better than those observed in routine formalin-fixed, paraffin-embedded tissue. Immunostaining profiles allowed distinction of tumour subtypes. Antigen retrieval techniques could be avoided in all cases.〈section xml:id="abs1-3"〉〈title type="main"〉ConclusionsApplication of the microbiopsy technique in routine cytology smears containing microbiopsies is helpful, particularly in those cases in which the diagnosis is not clear on the basis of the cytology smear and in cases in which there are not enough cytology slides for immunohistochemical examination.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-2559.1998.00476.x
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