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  • 1
    Digitale Medien
    Digitale Medien
    Establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 33 (2000), S. 0 
    Details
    ISSN: 1574-6941
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: In a field release experiment, rifampicin resistant mutants of two antagonistic plant-associated bacteria were used for seed tuber inoculation of transgenic T4 lysozyme expressing potatoes, transgenic control potatoes and non-transgenic parental potatoes. The T4 lysozyme tolerant Pseudomonas putida QC14-3-8 was originally isolated from the tuber surface (geocaulosphere) of T4 lysozyme producing plants and showed in vitro antibacterial activity to the bacterial pathogen Erwinia carotovora ssp. atroseptica. The T4 lysozyme sensitive Serratia grimesii L16-3-3 was originally isolated from the rhizosphere of parental potatoes and showed in vitro antagonism toward the plant pathogenic fungus Verticillium dahliae. The establishment of the inoculated bacteria in the rhizosphere and geocaulosphere of the different plant lines was monitored over one growing season to assess the effect of T4 lysozyme produced by transgenic potato plants on the survival of both inoculants. Both introduced isolates were able to colonize the rhizo- and geocaulosphere of transgenic plants and non-transgenic parental plants, and established in the rhizosphere at levels of ca. log10 5 colony forming units g−1 fresh weight of root. During flowering of plants, significantly more colony counts of the T4 lysozyme tolerant P. putida were recovered from transgenic T4 lysozyme plants than from the transgenic control and the parental line. At this time, the highest level of T4 lysozyme (% of total soluble protein) was detected. Effects of the inoculants on the indigenous microbial community were monitored by analysis of PCR-amplified fragments of the 16S rRNA genes of the whole bacterial community after separation by denaturing gradient gel electrophoresis (DGGE). At any sampling time, the DGGE pattern of rhizosphere and geocaulosphere communities did not show differences between the inoculated and non-inoculated potatoes. Neither of the introduced strains became a dominant member of the bacterial community. This work was the first approach to assess the establishment of plant growth promoting rhizobacteria and potential biocontrol agents on transgenic plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6941.2000.tb00725.x
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  • 2
    Digitale Medien
    Digitale Medien
    Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum (1990)
    Springer
    Plant molecular biology 15 (1990), S. 281-293 
    Details
    ISSN: 1573-5028
    Schlagwort(e): monoclonal antibody ; multimeric foreign protein ; self-assembly ; signal peptide ; transgenic plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Immunoglobulin light and heavy chains are synthesized in mammalian cells as precursors containing a signal peptide. Processing and assembling result in formation of active antibodies. Chimeric genes have been made containing the coding sequence of the barley α-amylase signal peptide which has been fused to cDNAs coding for either the mature light or the mature heavy chain of a monoclonal antibody. A plasmid was constructed linking both chimeric genes under the control of plant active promoters in an expression cassette. This DNA fragment was stably integrated into the genome of Nicotiana tabacum by Agrobacterium tumefaciens mediated gene transfer. Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies. By electron microscopic immunogold labeling, the presence of assembled antibody could be detected within the endoplasmic reticulum. Affinity chromatography indicated biological activity of the assembled immunoglobulin produced in plant cells. Unexpectedly, a significant amount of assembled antibodies was found within chloroplasts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00036914
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  • 3
    Digitale Medien
    Digitale Medien
    Can lysozymes mediate antibacterial resistance in plants? (1993)
    Springer
    Plant molecular biology 23 (1993), S. 209-214 
    Details
    ISSN: 1573-5028
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00021432
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  • 4
    Digitale Medien
    Digitale Medien
    Genetic engineering for resistance to bacteria in transgenic plants by introduction of foreign genes (1996)
    Springer
    Molecular breeding 2 (1996), S. 297-305 
    Details
    ISSN: 1572-9788
    Schlagwort(e): transgenic plants ; resistance ; phytopathogenic bacteria ; plant breeding ; genetic engineering ; potato ; tobacco
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00437908
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  • 5
    Digitale Medien
    Digitale Medien
    A plant transformation vector with a minimal T-DNA II. Irregular integration patterns of the T-DNA in the plant genome (1998)
    Springer
    Plant molecular biology 37 (1998), S. 581-585 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Agrobacterium - plant transformation - rearrangements - T-DNA integration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A minimal T-DNA binary vector was used for Agrobacterium-mediated transfer of a chimeric T4 lysozyme gene located next to the left border, and transgenic potato plants which expressed T4 lysozyme protein were identified and further analysed. Frequent rearrangements of T4 lysozyme transgenes were detected. A vector derivative containing two matrix associated regions (MARs) flanking its multiple cloning site was constructed. In transgenic potato plants, reduced variability in gene expression due to position effects was detected. When either the donor vector contained MAR sequences, or when vector pPCV701 which contains a pBR322 fragment next to the left border were used, only relatively few rearrangements were observed. However, when the T4 lysozyme gene was driven by a CaMV 35S promoter modified by multiplied enhancer region carrying either 2 or 4 elements, frequent rearrangements were again obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006035500546
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  • 6
    Digitale Medien
    Digitale Medien
    Potato tubers as a biofactory for recombinant antibodies (1998)
    Springer
    Molecular breeding 4 (1998), S. 313-319 
    Details
    ISSN: 1572-9788
    Schlagwort(e): antibody ; endoplasmic reticulum ; phytofarming ; potato ; production ; scFv
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 °C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009676832273
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  • 7
    Digitale Medien
    Digitale Medien
    A plant transformation vector with a minimal T-DNA (1994)
    Springer
    Transgenic research 3 (1994), S. 138-140 
    Details
    ISSN: 1573-9368
    Schlagwort(e): transformation vector ; Agrobacterium tumefaciens ; plant transformation ; T-DNA ; nptII gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plant transformation, viaAgrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during field testing of the transgenic plants or even more in the case of commercialization. The aim of this study was to produce a plant transformation vector which only contains a selectable and screenable marker gene and a multiple cloning site for insertion of promoter::foreign gene::terminator cassettes from other plasmids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01974093
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