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  • 1
    Digitale Medien
    Digitale Medien
    Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga Chlamydomonas Reinhardtii (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 78 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Using a biolistic device built here and based on the principle of the device described by Klein et al. (1987). we have reproducibly obtained transformants of Chlamydomonas reinhardtii. The reproducibility of the method has allowed us to examine the maintenance and expression of cloned DNA fragments introduced into C. Reinhardtii.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb02089.x
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  • 2
    Digitale Medien
    Digitale Medien
    Removal of antibiotic resistance genes from transgenic tobacco plastids (2000)
    [s.l.] : Nature America Inc.
    Nature biotechnology 18 (2000), S. 1172-1176 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] Removal of antibiotic resistance genes from genetically modified (GM) crops removes the risk of their transfer to the environment or gut microbes. Integration of foreign genes into plastid DNA enhances containment in crops that inherit their plastids maternally. Efficient plastid transformation ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/81161
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  • 3
    Digitale Medien
    Digitale Medien
    Characterization of transcribed dispersed repetitive DNAs in the nuclear genome of the green alga Chlamydomonas reinhardtii (1989)
    Springer
    Current genetics 16 (1989), S. 165-176 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Chlamydomonas reinhardtii ; Repetitive DNA ; Transcription-organization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Four cDNAs (cDNAs 1–4),162,338,321 and 167 by in size, that contain repetitive DNA sequences, were isolated from C. reinhardtii. cDNAs 1, 2 and 3 hybridized to multiple transcripts in poly A+ RNA. Each of the four repeat families is comprised of an extremely heterogeneous population of interspersed nuclear DNA sequences most of which are less than 0.5 kbp in size. A large number of restriction fragment length polymorphisms were uncovered by using cDNAs 1 and 2 as hybridization probes. cDNA2 was compared to two different genomic DNA sequences: the first sequence was complementary to a central 136 bases of cDNA2, which is bordered by a 15-bp imperfect direct repeat; the second sequence lacks a poly-dA tail, but is otherwise colinear along its entire length with cDNA2. This suggests that some members of the cDNA2 repeat family contain signals for polyadenylation. The majority of accumulated transcripts that hybridize to cDNA2 have the same 5′–3′ orientation as cDNA2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00391473
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  • 4
    Digitale Medien
    Digitale Medien
    Large arrays of tandemly repeated DNA sequences in the green alga Chlamydomonas reinhardtii (1993)
    Springer
    Chromosoma 102 (1993), S. 500-507 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract We describe the characterization of tandemly repeated DNA sequences, which resemble the satellite DNA sequences of multicellular eucaryotes, in the unicellular green alga Chlamydomonas reinhardtii. Restriction enzymes that cleave C. reinhardtii DNA relatively frequently produce a number of high molecular weight DNA fragments in addition to the bulk of low molecular weight DNA fragments. pTANC 1.5 contains a 1.5 kb Sau3A fragment cloned from one of these large bands. pTANC 1.5 hybridized to at least three large arrays (200 to 700 kb) of tandemly repeated DNA sequences in the cell-wall-deficient strain cw1.5. These arrays are composed of repeat units that are each cleaved once by BamHl into bands of 1.5, 1.9, 2.0 and 2.5 kb in size. The copy numbers of the 1.5, 1.9, 2.0 and 2.5 kb Bamhl bands vary between different C. reinhardtii strains. Chlamydomonas smithii and a number of C. reinhardtii strains are deficient in all four BamHl bands. Genetic analysis of wild-type strain 137c, which is deficient in the 2.0 kb BamHl band, indicates that the 1.5, 1.9 and 2.5 kb BamHl bands derive from at least five loci. The 1.5, 1.9 and 2.5 kb repeat units are not extensively interspersed with each other in strain 137c. Pulsed-field gel electrophoresis of intact C. reinhardtii chromosomes indicates that TANC arrays are present on more than one chromosome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00357106
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  • 5
    Digitale Medien
    Digitale Medien
    Deleted forms of plastid DNA in albino plants from cereal anther culture (1985)
    Springer
    Current genetics 9 (1985), S. 671-678 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Plastid DNA ; Barley ; Anther culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Wheat and barley albino plants derived from anther culture contain plastid genomes which have suffered deletion. DNA molecules of the size of unit genomes exist in these plants. In some cases these may be linear genomes. In all cases a region near the T8 fragment of barley or the corresponding region of the wheat plastid genome has been retained. This region may therefore represent sequences sufficient for replication.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00449820
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  • 6
    Digitale Medien
    Digitale Medien
    A transposon-like sequence with short terminal inverted repeats in the nuclear genome of Chlamydomonas reinhardtii (1995)
    Springer
    Plant molecular biology 28 (1995), S. 437-442 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Chlamydomonas ; transposon-like sequence ; nuclear genome
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A 1.2 kb DNA sequence, flanked by a potential seven base target-site duplication, was found inserted into a TOC1 transposable element from Chlamydomonas reinhardtii. The insertion sequence, named TOC2, is a member of a family of repeated DNA sequences that is present in all the C. reinhardtii strains tested. It resembles class II transposable elements: it possesses short 14 bp imperfect terminal repeats that begin AGGAGGGT, and sub-terminal direct repeats located within 250 bp of the termini. No large open reading frames were found. The terminal bases and length of target-site duplication are important in classifying transposable elements. On this basis TOC2 does not fall readily into existing families of class II transposable elements found in plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020392
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  • 7
    Digitale Medien
    Digitale Medien
    Structure, evolution and expression of the mitochondrial ADP/ATP translocator gene from Chlamydomonas reinhardtii (1993)
    Springer
    Molecular genetics and genomics 237 (1993), S. 134-144 
    Details
    ISSN: 1617-4623
    Schlagwort(e): ADP/ATP translocator ; Chlamydomonas ; Codon usage ; Heat shock ; Mitochondria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The first AUG in the Chlamydomonas reinhardtii ADP/ATP translocator (CRANT) mRNA initiates an open reading frame (ORF) which is very similar (51–79% amino acid identity) to other ANT proteins. In contrast to higher plants, no evidence for a long amino-terminal extension was obtained. The 5′ non-transcribed region of the single-copy CRANT gene contains sequence motifs present in other C. reinhardtii nuclear genes. Four introns, whose positions are not conserved in other ANT genes, interrupt the protein coding region. A short heat shock specifically reduces CRANT mRNA levels. CRANT mRNA levels were unaffected by a mutation in photosynthesis. In a dark/light regime CRANT mRNA levels are high in the dark phase and low in the early light phase. Data on translation initiation sites, splice junctions and the codon preferences of C. reinhardtii nuclear genes were compiled. With the exception of two rare codons, ACA and GGA, the CRANT gene exhibits the biased codon usage of C. reinhardtii nuclear genes that are highly expressed during normal vegetative growth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00282794
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  • 8
    Digitale Medien
    Digitale Medien
    Nif gene transfer and expression in chloroplasts: Prospects and problems (1997)
    Springer
    Plant and soil 194 (1997), S. 193-203 
    Details
    ISSN: 1573-5036
    Schlagwort(e): chloroplast ; genetic engineering ; nif genes ; nitrogenase ; plant transformation ; plastid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The engineering of plants capable of fixing their own nitrogen is an extremely complex task, requiring the co-ordinated and regulated expression of 16 nif genes in an appropriate cellular location. We suggest that plastids may provide a favourable environment for nif gene expression provided that the nitrogenase enzyme can be protected from oxygen damage. Using the non-heterocystous cyanobacteria as a model, we argue that photosynthesis could be temporally separated from nitrogen fixation in chloroplasts by restricting nitrogenase synthesis to the dark period. We report preliminary data on the introduction and expression of one of nitrogenase components, the Fe protein, in transgenic tobacco and Chlamydomonas reinhardtii. Finally we discuss potential avenues for further research in this area and the prospects for achieving the ultimate goal of expressing active nitrogenase in cereal crops such as rice.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1004296703638
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