Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Physiologia plantarum
43 (1978), S. 0
ISSN:
1399-3054
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
Nuclei were isolated from protoplasts obtained from Parthenocissus tricuspidata Crown Gall callus tissues. The effect of various isolation procedures, detergent or ultrasonication, on yield and quality of nuclei was studied. A standard procedure, based on the use of 5 × 10−3% Triton × 100 — 6% PVP — 20% glycerol, may be carried out in 30 min and gives 80 to 90% yield of nuclei in which RNA polymerase activity is retained.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1399-3054.1978.tb02564.x
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