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  • 1
    Digitale Medien
    Digitale Medien
    Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA (1994)
    Springer
    Archives of microbiology 161 (1994), S. 489-494 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Key words:Candida albicans– Dimorphism – Cell wall – Mannoprotein – EDTA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract. Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 °C in EDTA-free medium. High-molecular-weight mannoproteins (HMWM) that are mycelium-specific wall components, and particularly a 260-kDa species (HMWM-260), were absent in the wall of cells grown under germination conditions in the presence of EDTA. Synthesis of the HMWM-260 species was not inhibited but its incorporation (secretion) into the wall structure seemed to be blocked in EDTA-treated cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00307769
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  • 2
    Digitale Medien
    Digitale Medien
    Separation of chitosomes and secretory vesicles from the “slime” variant of Neurospora crassa (1987)
    Springer
    Archives of microbiology 149 (1987), S. 156-162 
    Details
    ISSN: 1432-072X
    Schlagwort(e): “Slime” variant ; Neurospora ; Chitosomes ; Chitin synthetase ; Secretory vesicles ; Invertase ; Phosphatase ; Membranes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cells from the “slime” variant of Neurospora crassa were broken in isotonic conditions by use of triethanolamine buffer plus EDTA. After removal of large membranous structures by low-speed centrifugation, chitosomes and secretory vesicles were separated by means of gel filtration, precipitation of membranous contaminants with Concanavalin A, and centrifugation in sucrose or glycerol gradients. Polypeptidic composition of fractions enriched in secretory vesicles or chitosomes was found to be distinct. By these criteria we concluded that chitosomes and secretory vesicles represent different populations of microvesicles. Both microvesicular populations appeared free of endoplasmic reticulum and vacuolar contaminants as demonstrated by determination of appropriate enzymatic markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425082
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  • 3
    Digitale Medien
    Digitale Medien
    Characterization of a proteinaceous extracellular coat synthesized by the “slime” variant of Neurospora crassa (1989)
    Springer
    Archives of microbiology 152 (1989), S. 25-32 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Neurospora crassa ; “Slime” variant ; Cell coat ; Exocellular proteins ; Glycoproteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cells of the “slime” strain of Neurospora crassa synthesize a coherent extracellular material which remains attached to the cell surface, but is released into the liquid medium by shaking. The material was purified and studied by different criteria. By electron microscopy it appears as long wavy sheets which strongly bind concanavalin A, but not wheat germ agglutinin, and maintain their integrity in the absence of structural polysaccharides. Analysis of the purified material revealed that it was free of contaminating membranes; it contained more than 70% protein, 1% neutral sugars (glucose, mannose, fucose and galactose), less than 2% lipids and ca. 4% not-characterized hexosaminelike compounds. Its polypeptide pattern as determined by PAGE was complex. The significance of this material is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447007
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  • 4
    Digitale Medien
    Digitale Medien
    Self-assembly properties of the proteinaceous coat secreted by the “slime” variant of Neurospora crassa (1989)
    Springer
    Archives of microbiology 152 (1989), S. 33-38 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Neurospora crassa ‘Slime” variant ; Cell coat ; Glycoproteins ; Self-assembling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The proteinaceous extracellular material (PEM) synthesized by the cells of the ‘slime” strain of Neurospora crassa (see Martinez et al. 1989) was solubilized by treatment with urea or guanidine. Removal of these chemicals by dialysis, caused reassembly of the solubilized proteins into material with the same microscopic appearance as the original PEM. Polypeptide patterns from both native and reassembled structures were identical. Dialysis-mediated reassembly of the solubilized proteins appeared to be dependent on both concentration of the soluble macromolecules and time. Gel chromatography of PEM solubilized with different agents revealed two discrete populations of complexes with molecular masses of 1,500 and 500 kDa respectively. These were able to reassemble into lamellar structures with a variable degree of efficiency.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447008
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