ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Abstract: We examined the changes in the molecular species of brain ethanolamine glycerophospholipids of monkeys fed diets containing widely ranging amounts of n-3 fatty acids. Two groups of rhesus monkeys were fed pre- and postnatally either a control diet (soy oil; containing 8% of fatty acids as 18:3n-3) or a deficient diet (safflower oil; containing 〈0.3% 18:3n-3). The brains of these animals were analyzed at 22 months of age. A third group of monkeys was fed the safflower oil diet to 22 months of age and then switched to a fish oil diet (28% long-chain n-3 fatty acids) for 1–2 years before autopsy. The molecular species of the diacyl, alkylacyl, and alkenylacyl ethanolamine glycerophospholipids from frontal cortex were separated by HPLC. A total of 24 molecular species were identified. Fatty acids in the sn-2 position differed markedly among the diet groups, but the sn-1 position always contained only 16:0, 18:0, or 18:1. In the diacyl subclass of the control brain, the n-3 molecular species represented 41% of total and the n-6 species 45%, whereas in the deficient brain the n-3 molecular species decreased to 9% and n-6 molecular species increased to 77%. The fatty acid 22:5n-6 did not replace 22:6n-3 in a symmetrical fashion in the molecular species of the deficient brain. In the brains of the fish oil-fed monkeys, the n-3 molecular species amounted to 61% and n-6 molecular species were reduced to 25%. The species 18:1–22:6, 16:0–22:6, and 18:0–22:6 generally changed proportionally in response to diet. However, 18:1–20:4, 16:0–20: 4, and 18:0–20:4 responded differently. The fish oil diet led to an increase in the proportion of 18:1–20:4 in the alkenylacyl subclass, whereas 16:0–20:4 and 18:0–20:4 decreased. Thus, total species containing sn-1 18:1 increased at the expense of sn-1 16:0 in the fish oil animals. Regardless of diet, each subclass of ethanolamine glycerophospholipid showed a strikingly different ratio of sn-1 16:0 to 18:0 to 18:1 for a given sn-2 fatty acid. In conclusion, the different diets had profound qualitative and quantitative effects on the molecular species of brain phospholipids, and these changes have implications for possible functional changes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1990.tb03125.x
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