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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 24 (2000), S. 0 
    ISSN: 1574-6976
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: In the last decade, a variety of ribosomally synthesized antimicrobial peptides or bacteriocins produced by lactic acid bacteria have been identified and characterized. As a result of these studies, insight has been gained into fundamental aspects of biology and biochemistry such as producer self protection, membrane–protein interactions, and protein modification and secretion. Moreover, it has become evident that these peptides may be developed into useful antimicrobial additives. Class IIa bacteriocins can be considered as the major subgroup of bacteriocins from lactic acid bacteria, not only because of their large number, but also because of their activities and potential applications. They have first attracted particular attention as listericidal compounds and are now believed to be the next in line if more bacteriocins are to be approved in the future. The present review attempts to provide an insight into general knowledge available for class IIa bacteriocins and discusses common features and recent findings concerning these substances.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Two Pseudomonas aeruginosa genes, termed phaJ1Pa and phaJ2Pa, homologous to the Aeromonas caviae (R)-specific enoyl-CoA hydratase gene (phaJAc) were cloned using a PCR technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (PHA) synthesis from β-oxidation cycle. Two expression plasmids for phaJ1Pa and phaJ2Pa were constructed and introduced into Escherichia coli DH5α strain. The recombinants harboring phaJ1Pa or phaJ2Pa showed high (R)-specific enoyl-CoA hydratase activity with different substrate specificities, that is, specific for short chain-length enoyl-CoA or medium chain-length enoyl-CoA, respectively. In addition, co-expression of these two hydratase genes with PHA synthase gene in E. coli LS5218 resulted in the accumulation of PHA up to 14–29 wt% of cell dry weight from dodecanoate as a sole carbon source. It has been suggested that phaJ1Pa and phaJ2Pa products have the monomer-supplying ability for PHA synthesis from β-oxidation cycle.
    Materialart: Digitale Medien
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  • 3
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A study of the feasibility of utilizing palm oil waste as a substrate for acetonebutanol-ethanol fermentation by Clostridium saccharoperbutylacetonicum N1-4 and, hence, to reduce the biochemical oxygen demand (BOD) of waste disposal was carried out. Among the two types of palm oil waste tested, separator sludge was the better substrate and it was able to support production of solvents by C. saccharoperbutylacetonicum N1-4 without a need for any mineral supplements. Enzymatic hydrolysis catalyzed by cellulase prior to fermentation was found to increase the yield of butanol by 75% (from 2.47g/l to 4.37g/l) and decreased the BOD by 66% (from 26,500 ppm to 8,900 ppm).
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Biotechnology letters 14 (1992), S. 599-604 
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The growth, substrate utilisation and L-lactate production ofLactococcus lactis IO-1 were examined on xylose, and glucose and xylose media. The yield of lactate on xylose was 0.47 g lactate/g xylose at an initial xylose concentration of 51.2 g/l and the μmax was 0.72 h−1. Xylose cultures were more susceptible to lactate inhibition than were glucose cultures but showed similar kinetic behaviour. The organism was capable of complete sugar utilisation when grown on a mixture of 20 g/l xylose and 20 g/l glucose and synthesised 0.66 g lactate/g sugar.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1573-6784
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A method based on density gradient centrifugation for the accurate and rapid determination of concentrations of intracellular metabolites was developed. The new method was applied to determination of intracellular levels of lactate during lactate fermentation and of intracellular levels of glutamate during glutamate fermentation. The method gave satisfactory results, showing good reproducibility and reliability with a probability of 95%. This method will allow basic information to be obtained about the transport of metabolites from within cells to the culture broth and about dynamic changes in metabolism.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Biotechnology techniques 11 (1997), S. 537-541 
    ISSN: 1573-6784
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Culture systems involving the use of one or two fermenters in series, with cell recycle employing hollow fiber filter modules to achieve high cell densities, were developed. Dilution rates of about 0.6 h -1 and ethanol productivity values of about 25 g/l h -1 , with residual glucose at 0.9 g/l, could be achieved using the system employing one fermenter.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Biotechnology letters 18 (1996), S. 1113-1118 
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Optimization of substrate feed for continuous production of lactic acid by the homofermentative bacterium, Lactococcus lactis IO-1, in glucose medium was investigated. A pH-dependent feed with two pH set-points, a lower set-point for neutralization with alkali and an upper set-point for substrate feed, proved better than continuous substrate feed with one pH set-point for neutralization with alkali only. Built-in electrodialysis with a cell-recycling system was tested and high cell density was achieved as a result of the use of enriched medium. However, specific lactate productivity in this system was not satisfactorily high. pH-dependent feed was combined with turbidity control and a cell recycling. With this system, we achieved high specific lactate productivity of 2 g (g-cell)-1 h-1 at a dilution rate of 0.5 h-1, a dry cell weight of 5 g l -1, a level of lactate in the broth of 20 g l -1, and a concentration of glucose in the spent medium of about 5 gl -1.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The relative contributions of lactate inhibition and the generation of sterile (undividing) cells to the low xylose utilisation rate of Lactococcus lactis IO-1 was investigated. The lactate inhibition constant of xylose grown cells was shown to be 9.3 times more than that of glucose grown cells. However, the sterile cell production rate and LDH inactivation rate of the xylose cultures were at least 10 times less than the glucose cultures. Thus, it is suggested that the slower substrate consumption rate in xylose medium is caused mainly by the large inhibition constant for the end product.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1573-6776
    Schlagwort(e): Alcaligenes eutrophus ; biodegradable polyester ; pH-stat substrate feeding ; propionic acid ; P(3HB-co-3HV)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The feeding of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] by Alcaligenes eutrophus ATCC17697 was optimized using a fed-batch culture system. The concentration of propionic acid was maintained at 3 g l−1 as growth was inhibited by propionic acid in the broth. A pH-stat substrate feeding system was used in which propionic acid was fed automatically to maintain a pH of the culture broth at 7.0. By feeding a substrate solution containing 20% (w/v) propionic acid, 4.9% (w/v) ammonia water [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10] in cell growth phase, the concentration of propionic acid in the broth was maintained at 3 g l−1 giving a specific growth rate of 0.4 h−1. To promote P(3HB-co-3HV) production, two stage fed-batch culture which consisted of the stage for the cell growth and the stage for the P(3HB-co-3HV) accumulation was carried out. When the substrate solution whose C/N molar ratio was 50 was fed in P(3HB-co-3HV) accumulation phase, the cell concentration and the P(3HB-co-3HV) content in the cells reached 64 g l−1 and 58% (w/w) in 55.5 h, respectively.
    Materialart: Digitale Medien
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