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Digitale Medien

Application of capillary electrophoresis to amino acid sequencing of peptide (1995)

Kim, Nam Jung ; Kim, Jung Ho ; Lee, Kong-Joo

Weinheim : Wiley-Blackwell

Electrophoresis 16 (1995), S. 510-515

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Details

ISSN: 0173-0835

Schlagwort(e): Capillary electrophoresis ; Micellar electrokinetic chromatography ; Peptide sequencing ; Phenylthiohydantoin-amino acids ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Separations of twenty phenylthiohydantoin (PTH) amino acids, and amino acid derivatives, resulting from the Edman degradation of peptides and proteins, were optimized for peptide sequencing by capillary electrophoresis. Manual sequencing of angiotensin II was performed by Edman degradation and capillary electrophoresis of the PTH amino acid obtained after each cycle. The results were compared with those of an automated conventional protein sequencer. Interfacing capillary electrophoresis with Edman degradation provides an additional option for protein sequencing.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150160184

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