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Digitale Medien

Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures (1984)

Nakagawa, K. ; Konagai, A. ; Fukui, H. ; [weitere]

Springer

Plant cell reports 3 (1984), S. 254-257

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Details

ISSN: 1432-203X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400–800 mg/l) when 5–10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cells into the liquid medium, and an excess of berberine crystallized as its nitrate in the medium. When the cells were cultured in a modified LS medium containing 20 mM KNO3 and 40 mM NH4Cl in place of 20.6 mM NH4NO3 as nitrogen source, most of the alkaloid crystallized to form berberine chloride instead of nitrate. Minor alkaloids, thalifendine and magnoflorine, were also isolated from the medium and identified.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00269306

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