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  • 1
    Digitale Medien
    Digitale Medien
    Purification, serology and physico-chemical properties of a peanut mottle virus isolate from India (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 32 (1983), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: A procedure developed for the purification of peanut mottle virus (PMV) isolated from peanuts in India yielded 30–40 mg of virus/kg of plant tissue. Purified virus sedimented as a single component of 151 S. The virus coat protein migrated as a single component in each of two polyacrylamide gel concentrations and had an estimated molecular weight of 34 000 daltons. The molecular weight of the nucleic acid was 3.10 × 106 daltons. In the precipitin ring interface test (PRIT) PMV showed a serological relationship with soybean mosaic virus (SMV). Using the double antibody sandwich form of ELISA, PMV was shown to be distantly related to adzuki bean mosaic (ABMV), amaranthus leaf mottle (ALMV), clover yellow vein (CYVV) viruses and SMV. Immunosorbent electron microscopy (ISEM) showed PMV to be closely related to ABMV, ALMV, CYVV and SMV. In PRIT, ELISA and ISEM tests no specific serological reaction was noted between PMV and antisera to groundnut eye spot, peanut green mosaic, pepper veinal mottle, potato virus Y, sugarcane mosaic and turnip mosaic viruses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3059.1983.tb01319.x
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  • 2
    Digitale Medien
    Digitale Medien
    Isolation and characterization of a potyvirus associated with bushy dwarf symptom in chickpea, Cicer arietinum, in India (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: A potyvirus that induced stunting and a characteristic bushy appearance at the apical region, due to proliferation of terminal branches with narrowed, reduced and deformed leaflets, was isolated from chickpea in India. The virus was sap-transmissible to 14 species of Chenopodiaceae, Leguminosae, Solanaceae and Malvaceae; Chenopodium amaranticolor was a good local lesion host. Virus particles, trapped by immunosorbent electron microscopy and stained with uranyl acetate, were 710 ×10 nm long. Purified virus preparations contained a single polypeptide species of 32,500 Da and one nucleic acid species of 3.1 · 106 Da. The virus was serologically related to soybean mosaic, azuki bean mosaic and peanut mottle viruses but not to clover yellow vein, pea seed-borne mosaic and bean yellow mosaic viruses.On the basis of these properties, the virus was identified as a previously undescribed potyvirus in chickpea, for which the name chickpea bushy dwarf virus is proposed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3059.1989.tb01446.x
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  • 3
    Digitale Medien
    Digitale Medien
    A plant rhabdovirus associated with peanut veinal chlorosis disease in India (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: A disease of peanut characterized by stunting of plants, veinal chlorosis, outward bending of leaflets, and proliferation of axillary buds has been observed in several parts of Peninsular India since 1977. The disease was restricted to peanut crops raised during the post-rainy season. A rhabdovirus was associated with this disease. This appears to be the first record of natural occurrence of a rhabdovirus in peanut.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3059.1989.tb01462.x
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  • 4
    Digitale Medien
    Digitale Medien
    Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 40 (1991), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3059.1991.tb02412.x
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  • 5
    Digitale Medien
    Digitale Medien
    Dynamics of Polymyxa graminis and Indian peanut clump virus (IPCV) infection on various monocotyledonous crops and groundnut during the rainy season (2002)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 51 (2002), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The progress of Indian peanut clump virus (Hyderabad isolate; IPCV-H) and its vector Polymyxa graminis in various monocotyledonous crops and groundnut was studied during the 1994, 1995 and 1996 rainy seasons in a naturally infested field in India. The roles of rainfall and temperature in the dynamics of infection by both the virus and its vector were analysed by exposing young seedlings for short periods in the field. Of the host crops studied, wheat, followed by barley, showed the highest virus incidence, although P. graminis was rarely observed in roots of wheat and was not detected in those of barley. The roots of maize, pearl millet and sorghum plants infected by P. graminis showed intense colonization by sporosori. IPCV accumulated in systemically infected maize plants; the sorghum and pearl millet cultivars studied showed a transient presence of IPCV-H. Rice was seldom infected by the virus and P. graminis was not detected in its roots. Groundnut was a systemic host for the virus, although during these experiments no P. graminis was found in its roots. Groundnut appeared to be susceptible to infection, mostly in the early stages of crop development, and the rate of IPCV-H transmission in groundnut seeds was highest (13%) for plants infected when young. The seed-transmission rate quickly decreased in plants showing symptoms 1 month after sowing. Time of infection had little influence on groundnut pod yield, which was always reduced by 〉60% in infected plants. There was some evidence that the quantity and distribution of rainfall influenced the incidences of IPCV-H and P. graminis: high rainfall resulted in high incidences of the virus and P. graminis, and a weekly rainfall of 14 mm was sufficient for P. graminis to initiate infection. Temperatures prevailing during the rainy season ranged from 23 to 30°C and were found to be conducive to natural virus transmission. These results suggest measures to be explored for controlling peanut clump disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-3059.2002.00752.x
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  • 6
    Digitale Medien
    Digitale Medien
    Peanut yellow spot virus is a member of a new serogroup of Tospovirus genus based on small (S) RNA sequence and organization (1998)
    Springer
    Archives of virology 143 (1998), S. 353-364 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary.  Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. The sequence of the S RNA was 2 970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5′-large ORF (1 440 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16–21% sequence identity and 42– 48% sequence similarity with other tospoviruses. A 3′ ORF (741 nucleotides) in the virus complementary (vc) sense encoded a 28.0 kDa protein that was identified as the nucleocapsid (N) gene based on immunoblot analysis of the {in vitro} expressed protein with PYSV polyclonal antiserum. The predicted N protein had 24–28% amino acid sequence identity and 44–51% sequence similarity with the members of other serogroups. In contrast to other tospoviruses, a third ORF (204 nucleotides) occurred in the vc strand, which could encode a protein with a predicted size of 7.5 kDa with two strong hydrophobic regions. The low degree of homology of N and NSs protein sequences with other serogroup members coupled with an additional ORF suggests that PYSV should be classified as a distinct species of the Tospovirus genus. This conclusion also is supported by the absence of serological cross reaction with other serogroups, and biological characteristics including thrips transmission, symptoms and host range.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s007050050291
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  • 7
    Digitale Medien
    Digitale Medien
    Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses (1996)
    Springer
    Archives of virology 141 (1996), S. 85-98 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3 057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A+U-rich intergenic region. One ORF (1 320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein. This protein was identified as the nucleocapsid (N) protein based on sequence similarities. Amino acid sequence comparison of N and NSs proteins revealed identities of 22–34% with the reported tospovirus isolates of serogroups I, II, and III, whereas it had 82–86% identity with viruses in serogroup IV, watermelon silver mottle virus (WSMV) and tomato isolate of peanut bud necrosis virus (PBNV-To). Two subgenomic RNA species detected in PBNV infected tissue corresponded to the predicted sizes (1.65 and 1.4 kb) of the NSs and N mRNAs. The data presented show conclusively that PBNV should be included in serogroup IV, along with WSMV and PBNV-To.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01718590
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  • 8
    Digitale Medien
    Digitale Medien
    Evidence that whitefly-transmitted cowpea mild mottle virus belongs to the genus Carlavirus (1998)
    Springer
    Archives of virology 143 (1998), S. 769-780 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary.  Two strains of whitefly-transmitted cowpea mild mottle virus (CPMMV) causing severe (CPMMV-S) and mild (CPMMV-M) disease symptoms in peanuts were collected from two distinct agro-ecological zones in India. The host-range of these strains was restricted to Leguminosae and Chenopodiaceae, and each could be distinguished on the basis of symptoms incited in different hosts. The 3′-terminal 2500 nucleotide sequence of the genomic RNA of both the strains was 70% identical and contains five open reading frames (ORFs). The first three (P25, P12 and P7) overlap to form a triple gene block of proteins, P32 encodes the coat protein, followed by P12 protein located at the 3′ end of the genome. Genome organization and pair-wise comparisons of amino acid sequences of proteins encoded by these ORFs with corresponding proteins of known carlaviruses and potexviruses suggest that CPMMV-S and CPMMV-M are closely related to viruses in the genus Carlavirus. Based on the data, it is concluded that CPMMV is a distinct species in the genus Carlavirus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s007050050328
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  • 9
    Digitale Medien
    Digitale Medien
    Serological comparison of tospovirus isolates from Taiwan and India with impatiens necrotic spot virus and different tomato spotted wilt virus isolates (1993)
    Springer
    Archives of virology 130 (1993), S. 237-250 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Tospovirus isolates from tomato and watermelon in Taiwan and from peanut in India reacted neither with antibodies to tomato spotted wilt virus (TSWV), nor with antibodies to impatiens necrotic spot virus (INSV), in ELISA and in electroblot immunoassays. Additionally, all three tospovirus isolates had an N protein with a molecular weight of 32 kDa as compared to 29 kDa for the N protein to TSWV and INSV. The observed physicochemical and serological differences suggest the generation of a new, third species in the genus tospovirus for which we propose the name groundnut bud necrosis virus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01309657
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  • 10
    Digitale Medien
    Digitale Medien
    The coat protein of Indian peanut clump virus: relationships with other furoviruses and with barley stripe mosaic virus (1994)
    Springer
    Archives of virology 134 (1994), S. 271-278 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The 5′-most open reading frame of the c.4kb RNA-2 of Indian peanut clump furovirus (IPCV) encodes a protein of 208 amino acids. This protein is thought to be the coat protein of IPCV because its amino acid composition and Mr closely resemble those reported for IPCV coat protein and because its amino acid sequence is 61% identical to that of the coat protein of peanut clump virus (PCV) from West Africa. The extent of the sequence identity between IPCV and PCV coat proteins confirms previous conclusions that the viruses are distinct rather than strains of one virus. The sequences of the coat proteins of IPCV and PCV were between 18% and 26% identical to those of other furoviruses and those of unrelated tobamoviruses and tobraviruses. In contrast, the coat protein sequences were 37% (IPCV) and 36% (PCV) identical to that of the coat protein of barley stripe mosaic hordeivirus (BSMV). This similarity between the coat proteins of viruses from different groups (=genera) is unusual but is consistent with previous reports of sequence relatedness in various genes between certain furoviruses and BSMV.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01310566
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