ISSN:
0021-9541
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Medizin
Notizen:
Expression of c-fos is induced by a number of signals in several cell systems. Although the exact function of the c-fos product is unknown, it has been implicated to be of importance for both cell growth and differentiation (Verma and Sassone-Corsi, 1987). To analyze how c-fos expression relates to in vitro myogenic differentiation, the kinetics of c-fos mRNA expression during spontaneous in vitro differentiation of L6J1 myoblasts was examined; c-fos transcripts were most abundant at day 4 of the differentiation process. Multinucleated myotubes and expression of α-actin and myosin heavy chain (MHC) mRNA appeared later, at day 6 or 7, and increased to maximal levels after 10 days in culture. To analyze further the relation between c-fos expression and L6J1 myogenic differentiation, L6J1 myoblasts were transfected with expression vectors containing the murine c-fos gene driven by a metallothionein promoter. The growth rate of c-fos-transfected L6J1 cells did not differ from that of control cells. However, formation of myotubes was significantly reduced in c-fos-transfected L6J1 cultures compared with neo-transfected controls. Myotube formation and expression of the myogenic markers α-actin and MHC were reduced in subclones expressing high levels of c-fos, but not in subclones with lower levels of c-fos expression. These results indicate that a marked elevation of c-fos expression at least partially inhibits L6J1 myogenic differentiation.
Zusätzliches Material:
1 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jcp.1041390204
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