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  • 1
    Digitale Medien
    Digitale Medien
    Purification and characteristics of photoreceptor light-activated guanosinetriphosphatase (1981)
    s.l. : American Chemical Society
    Biochemistry 20 (1981), S. 7068-7074 
    Details
    ISSN: 1520-4995
    Quelle: ACS Legacy Archives
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/bi00528a003
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  • 2
    Digitale Medien
    Digitale Medien
    Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus (1999)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 345-346 
    Details
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310 K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21 with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 Å and β = 98.3°.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0907444998008130
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  • 3
    Digitale Medien
    Digitale Medien
    Midkine, a new neurotrophic factor, is present in glial cytoplasmic inclusions of multiple system atrophy brains (2000)
    Springer
    Acta neuropathologica 100 (2000), S. 481-489 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Glial cytoplasmic inclusion ; Granule-coated fibril ; Midkine ; Multiple system ; atrophy ; Oligodendrocyte
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The glial cytoplasmic inclusion (GCI) is a histological hallmark for multiple system atrophy (MSA): these inclusions are found in oligodendrocytes and consist of abnormal granule-coated fibrils of approximately 24- to 40-nm diameter. To clarify the significance of the presence of midkine (MK) in these GCIs, we carried out immunohistochemical, electron and immunoelectron microscopical, and Western blot analyses of MSA brains using a monoclonal antibody against the C-terminal region of human MK. Immunohistochemically, most of the GCIs were intensely stained by the antibody to MK. Electron and immunoelectron microscopy showed that the GCIs were composed of MK-positive granule-coated fibrils that were essential constituents of these inclusions. No significant MK immunoreactivity was observed in oligodendrocytes, astrocytes and neurons of the normal control subjects. The presence of MK in MSA brain but not in normal brain was confirmed by Western blotting. Together with the fact that MK is associated with fetal morphogenesis during the midgestation period, the presence of MK immunoreactivity in oligodendroglial GCIs may suggest the existence of a repair mechanism on the basis of morphogenesis in the degenerated oligodendrocytes themselves as well as the affected neurons and their axons through the oligodendrocyte-axon-neuron relationship.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010000214
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  • 4
    Digitale Medien
    Digitale Medien
    Induction of apoptosis in cultured cells by extracts from shiitake (Lentinula edodes) mycelial culture broth (2000)
    Springer
    Mycoscience 41 (2000), S. 623-631 
    Details
    ISSN: 1618-2545
    Schlagwort(e): apoptosis ; caspase-3 ; E2F factor ; Lentinula edodes ; mycelial culture broth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Extracts fromshiitake (Lentinula edodes) mycelial culture broth, by an organic solvent ethyl acetate, inhibited the proliferation of cultured cells. At lower concentrations (1.25–15 μg/ml), this inhibition, measured by the MTT assay, was dose- and cell line-dependent. Inhibition of tumor cells, such as Caski, SiHa, HeLa, HP-1 and A375, byL. edodes-436 extracts was stronger than inhibition of normal cells (3T3). At 20 μg/ml, the extracts induced changes in cell shape, DNA-fragmentation and the activation of caspase-3. The extracts also inhibited the binding of E2F protein to its promoter. The results suggest that extracts ofL. edodes culture broth contain substances that have the ability to induce apoptosis in the cultured cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02460929
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  • 5
    Digitale Medien
    Digitale Medien
    Regulation factor for planarian regeneration (1995)
    Springer
    Hydrobiologia 305 (1995), S. 247-253 
    Details
    ISSN: 1573-5117
    Schlagwort(e): planarian ; regeneration ; regeneration factor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Planarian head extract was fractionated and the fractions were assayed for their effect on cultured cells and planarian regeneration. One fraction (molecular weight larger than 10 000; unadsorbable by DEAE-Sephadex, CM-Sephadex and Con A-Sepharose; and precipitable by ammonium sulfate) inhibited the growth of both Neuro 2a and PC-12 cell lines as well as planarian head- regeneration. This effect was specific for head-regeneration (regeneration of tails was not influenced), trypsin sensitive, reversible and stable after heat-treatment at 80° C for 30 min.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00036397
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  • 6
    Digitale Medien
    Digitale Medien
    Analysis of ploidy in a planarian by flow cytometry (1991)
    Springer
    Hydrobiologia 227 (1991), S. 175-178 
    Details
    ISSN: 1573-5117
    Schlagwort(e): planarian ; ploidy analysis ; flow cytometry ; mixoploidy ; propidium iodide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Flow cytometry (flow microfluorimetry) provides a quick means for analysis of ploidy in planarians. Nuclei from homogenized tissues of the freshwater planarian Dugesia japonica japonica Ichikawa et Kawakatsu were stained with propidium iodide and measured with an argon-laser flow cytometer to produce histograms of DNA content. Tissues from sexually mature individuals produced histograms with a 1n (haploid) peak but no 3n peak (triploid peak), whereas those from asexual individuals showed a 2n peak or a 3n peak or both, but no 1n peak. Thus, the 1n peak distinguished sexual individuals. Mixoploid individuals, i.e., mosaics with both diploid and triploid tissues, were identified by the presence of both a 2n peak and a 3n peak. The ratios of the heights of the 2n and 3n peaks from tissues in different parts of a single mixoploid individual were similar, suggesting that the diploid and triploid cells are homogeneously distributed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027599
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  • 7
    Digitale Medien
    Digitale Medien
    Inhibition of planarian regeneration by melatonin (1991)
    Springer
    Hydrobiologia 227 (1991), S. 31-40 
    Details
    ISSN: 1573-5117
    Schlagwort(e): planarian ; regeneration ; melatonin ; serotonin ; radioimmunoassay ; positional information
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Melatonin, which is a substance produced by the pineal body in vertebrates, inhibited regeneration in the planarian Dugesia japonica japonica Ichikawa et Kawakatsu. When decapitated planarians were maintained in a 1 mmol dm−3 solution of melatonin, formation of the head was retarded; formation of the eyes, however, was not disturbed. Similarly in animals from which the tail was cut, regeneration of the tail was retarded if the animals were kept in melatonin solution of 1 mmol dm−3. The effect was reversible once the melatonin was removed. Retardation of regeneration did not occur with similar application of three melatonin derivatives, serotonin hydrochloride, N-acetylserotonin, and 6-hydroxymelatonin. Melatonin endogenous to the planarian could be demonstrated by means of radio-immunoassay and was more abundant in the head region than other regions of the body. Melatonin, thus, appears to play a role in regulating regeneration in planarians and conceivably provides positional information in that process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027578
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  • 8
    Digitale Medien
    Digitale Medien
    Presence of rhodopsin-like proteins in the planarian head (1991)
    Springer
    Hydrobiologia 227 (1991), S. 93-94 
    Details
    ISSN: 1573-5117
    Schlagwort(e): planarian ; rhodopsin-like proteins ; anti-rhodopsin IgG
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The presence of rhodopsin-like protein was detected in the head of the freshwater planarian Dugesia japonica japonica Ichikawa et Kawakatsu by use of anti-frog-rhodopsin rabbit IgG. Two membrane proteins of molecular weights 65000 and 62000 were separated by sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and found to react with the anti-rhodopsin IgG. The antibody may be useful for monitoring regeneration of the planarian eye.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027587
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  • 9
    Digitale Medien
    Digitale Medien
    Preparation of monoclonal antibodies against planarian organs and the effect of fixatives (1995)
    Springer
    Hydrobiologia 305 (1995), S. 255-257 
    Details
    ISSN: 1573-5117
    Schlagwort(e): planarian ; monoclonal antibody ; fixing reagent
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Monoclonal antibodies were obtained against all part of the body of the planarian Dugesia japonica japonica except tthe nerve cord, though parts of the enteric canal were also non reactive. The effect of a variety of fixatives (acetone, Bouin, formalin) used on planarian tissues prior to screening with antibodies, was assessed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00036398
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  • 10
    Digitale Medien
    Digitale Medien
    Expression and purification of a recombinant human glycoprotein in mouse hybridoma SP2/0-AG14 cells (1997)
    Springer
    Biotechnology letters 19 (1997), S. 185-188 
    Details
    ISSN: 1573-6776
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The cDNA for human erythropoietin (hEPO) inserted into the mammalian expression vector BMCGSNeo was introduced into SP2/0-Ag14 cells and a transformant producing large amounts of hEPO was established. The recombinant hEPO in conditioned medium was purified by immunoaffinity and gel filtration column chromatographies. The purified hEPO had full in vitro biological activity, but low in vivo biological activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018328801615
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