ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
In this report we show that the two monoclonal anti-CD45 antibodies, EO-1 and FN-I26, potently inhibit G0 to G1 transition and S phase entry in human B cells stimulated with anti-μ and low molecular weight B-cell growth factor. Both antibodies were found to inhibit anti-μ-induced inositol phospholipid breakdown and c-myc mRNA induction. In contrast, EO-I and FN-I26 only partially inhibited the early anti-μ-induced increase in cytoplasmic Ca2+ levels, both in normal and in Ca2+-depleted medium. B-cell activation provoked by 12-O-tetradecanoylphorbol 13-acetate(TPA) was not inhibited by these antibodies, except when using high concentrations of EO-1. In addition, both antibodies were found to inhibit G1 entry induced by the anti-CD20 antibody IF5, which confers an activation of B cells without any detectable increase in [Ca2+]1 or in phospholipid metabolism. This indicates that alternative mechanisms in addition to the inhibition of polyphosphoinositide (PI) breakdown are involved in the inhibitory action of these antibodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1990.tb02809.x
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