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  • 1
    Electronic Resource
    Electronic Resource
    Differential Analysis by Various Staining Techniques of Structures present in Developing Spermatids of the Silkworm (1964)
    [s.l.] : Nature Publishing Group
    Nature 204 (1964), S. 1197-1198 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Testes of the fifth larval instar of silkworm were immersed for 1-2 h in 1 per cent osmium tetroxide or potassium permanganate buffered to pH. 7-4 with veronal-acetate buffer. When the specimens were fixed, they were dehydrated in a series of increasing concentrations of ethyl alcohol, and embedded ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2041197a0
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemistry and immunocytochemistry of atrial natriuretic polypeptide in porcine heart (1987)
    Springer
    Histochemistry and cell biology 86 (1987), S. 595-601 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific granules in porcine hearts were observed in atrial cardiocytes, Purkinje fibers, and transitional cells of the ventricle. These granule-containing cells were immunohistochemically stained by applying the avidin-biotin-peroxidase complex method using an antiserum against α-human atrial natriuretic polypeptide (ANP). Immunoelectron microscopy of sections stained using the immunogold method indicated that these specific granules are storage sites of ANP. Furthermore, an impulse-conducting system consisting of immunoreactive cells was clearly distinguishable from nonimmunoreactive ventricular cardiocytes. We conclude that specific-granule-containing cells, i.e., ANP-producing cells, are located in both the atrial walls and the ventricular impulse-conducting system. The presence of ANP may be correlated with impulse conduction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489553
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  • 3
    Electronic Resource
    Electronic Resource
    Masking of sperm maturation antigen by sialic acid in the epididymis of the mouse (1988)
    Springer
    Histochemistry and cell biology 90 (1988), S. 195-200 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sperm antigen expression during epididymal transit was examined in 4- to 16-week-old intact and castrated ICR mice, using the avidin-biotin complex (ABC) immunohistochemical method with monoclonal antibody T21 against a flagellar surface antigen. On untreated sections, the antigen was first expressed weakly on sperm in the proximal part of the corpus epididymis, and intraluminal components were stained in 4-week-old mice. Epididymal epithelial cells and their stereocilia, and cells in other reproductive organs were not stained. In contrast, on sections treated with neuramainidase, (1) the initial site of antigen appearance is a more proximal position in treated than in untreated sections, (2) stereocilia stained strongly, (3) the staining intensity of sperm and intraluminal components increased, and (4) some clear cells in the epithelium from the distal position of the caput to the corpus epididymis were stained. These results indicate that the antigen is produced by clear cells of the epididymal epithelium, that the antigenic determinant is masked initially by sialic acid residues, and that expression of the antigenic determinant on the sperm surface during epididymal maturation apparently involves desialylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492507
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis (1992)
    Springer
    Cell & tissue research 270 (1992), S. 459-468 
    Details
    ISSN: 1432-0878
    Keywords: Acrosome ; Equatorial segment of spermatozoa ; Transport pathway ; Spermiogenesis ; Monoclonal antibody ; Immuno-electron microscopy ; Mouse (BALB/c; CD-1; ICR) ; Rat (Wistar) ; Golden hamster-Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acrosome membranes and on the acrosome membranes at the equatorial segment. Immunoblotting after electrophoresis of spermatozoa from the cauda epididymidis has identified two immunoreactive bands: 38 kDa and 48 kDa in mouse, and 48 kDa in rat. During spermiogenesis in rat, this antigen is transported to the equatorial segment via a unique pathway, first appearing in some cisternae of the endoplasmic reticulum and in the Golgi apparatus of spermatids at around step 3. The antigen can further be found on the vesicles at thetrans-side of the Golgi apparatus, in the matrix of the head cap, and on the head cap membrane in step-4 to step-7 spermatids. The antigen appears to be concentrated at the equatorial segment during late spermiogenesis. Neither the (pro-)acrosomic granule nor the surrounding membrane are required in this pathway. This pathway can be termed the ‘Golgi-head cap tract’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00645047
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  • 5
    Electronic Resource
    Electronic Resource
    Extra-Golgi pathway of an acrosomal antigen during spermiogenesis in the rat (1992)
    Springer
    Cell & tissue research 270 (1992), S. 451-457 
    Details
    ISSN: 1432-0878
    Keywords: Intra-acrosomal protein ; Acrosome development ; Extra-Golgi pathway ; Spermiogenesis ; Immunoelectron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A monoclonal antibody (MC41) was produced that specifically recognizes a sperm acrosomal antigen of approximately 165000 dalton in the rat. Rat testis was examined using a pre-embedding immunoperoxidase technique to reveal the pathway of the MC41 antigen to the acrosome during spermiogenesis. The MC41 immunoreaction appeared in several organelles of spermatids in a stage-specific manner: (1) in the endoplasmic reticulum (ER) throughout spermiogenesis, (2) in the outer acrosomal membrane from steps 9 to 19, (3) as a weak immunoreaction in the vesicular structures in the acrosomal matrix from steps 11 to 17, and (4) as a strong immunoreaction in the acrosomal matrix especially at the terminal step of spermiogenesis (step 19). However, no immunoreaction was observed in the Golgi region throughout spermiogenesis. These results suggest that the pathway of the MC41 antigen leads firstly from the ER to the outer acrosomal membrane and secondly to the acrosomal matrix. This pathway does not involve the Golgi apparatus and is referred to as the “extra-Golgi pathway”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00645046
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  • 6
    Electronic Resource
    Electronic Resource
    Appearance of an intra-acrosomal antigen during the terminal step of spermiogenesis in the rat (1992)
    Springer
    Cell & tissue research 267 (1992), S. 203-208 
    Details
    ISSN: 1432-0878
    Keywords: Intra-acrosomal protein ; Monoclonal antibody ; Acrosome development ; Spermiogenesis ; Intracellular transport ; Testis ; Rat (Wistar) ; Mouse (ICR, BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a survey of sperm antigens in the rat, a new intra-acrosomal antigen was found using a monoclonal antibody MC41 raised against rat epididymal spermatozoa. The MC41 was immunoglobulin G1 and recognized spermatozoa from rat, mouse and hamster. Indirect immunofluorescence with MC41 specifically stained the crescent region of the anterior acrosome of the sperm head. Immuno-gold electron microscopy demonstrated that the antigen was localized within the acrosomal matrix. Immunoblot study showed that MC41 recognized a band of approximately 165000 dalton in the extract of rat sperm from the cauda epididymidis. Immunohistochemistry with MC41 demonstrated that the antigen was first detected in approximately step-2 spermatids, and distributed over the entire cytoplasmic region of spermatids from step 2 to early step 19. The head region became strongly stained in late step-19 spermatids and then in mature spermatozoa. Distinct immunostaining was not found in the developing acrosome of spermatids throughout spermiogenesis. These results suggest that the MC41 antigen is a unique intra-acrosomal antigen which is accumulated into the acrosome during the terminal step of spermiogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00302956
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  • 7
    Electronic Resource
    Electronic Resource
    A monoclonal antibody, MN13, that recognizes specifically a novel substance between the postacrosomal sheath and the overlying plasma membrane in the mammalian sperm head (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 29 (1991), S. 289-293 
    Details
    ISSN: 1040-452X
    Keywords: Postacrosomal region ; Cytoskeleton ; Ultrastructure ; Immunocytochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Monoclonal antibody MN13 raised against mouse spermatozoa specifically recognizes the postacrosomal region of the sperm head in several mammalian species. Colloidal gold-immunoelectron microscopy of demembranated mouse spermatozoa indicated that the antigen is associated with the outer layer of the periodic substructure apparently linking the postacrosomal sheath to the overlying plasma membrane. The antigen recognized by MN13 may cotribute to the intimate association of the postacrosomal sheath with the overlying plasma membrane.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080290312
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  • 8
    Electronic Resource
    Electronic Resource
    A rat sperm flagellar surface antigen that originates in the testis and is expressed on the flagellar surface during epididymal transit (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 32 (1992), S. 399-408 
    Details
    ISSN: 1040-452X
    Keywords: Monoclonal antibody ; MC31 ; Spermatogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We identified a rat sperm flagellar surface antigen using an IgG1 monoclonal antibody (MC31) against rat epididymal sperm. Avidin-biotin-peroxidase immunohistochemistry demonstrated that the antigen was first expressed in the cytoplasm of early primary spermatocytes, then gradually became restricted to the principal piece of the sperm flagellum during spermatogenesis. However, when the sperm reached the corpus epididymidis, the antigen was expressed on the surface of both the principal piece and the midpiece of the flagellum. The epithelial cells of the epididymis were not stained with MC31. Immunogold electron microscopy showed that the antigen was present on the surface of the sperm flagellar plasma membrane. Immunoblotting of Triton X-100 extracts of epididymal sperm after one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions demonstrated that MC31 detected a major antigen of 26,000-28,000 daltons (26-28K). Two-dimensional isoelectric focusing and SDS-PAGE indicated that the 26-28K antigen had an isoelectric focusing point (pl) of 5.8-5.3; minor antigens were also detected from 26K (pl 5.8) to 35K (pl 5.0). These results indicate that the antigen recognized by MC31 is an acidic 26-35K protein that originates in the testis, is integrated into the sperm flagellar plasma membrane of the principal piece during spermatogenesis, and then is expressed on the entire flagellar surface during epididymal transit. © 1992 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080320415
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