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  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity of bacteria associated with the hindgut of the terrestrial crustacean Porcellio scaber (Crustacea: Isopoda) (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 40 (2002), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Molecular approaches were used to examine the genetic diversity of bacteria associated with the gut wall of the terrestrial isopod Porcellio scaber and to determine whether an autochthonous microflora exists in the P. scaber hindgut. 16S ribosomal genes were amplified from the total DNA isolated from thoroughly washed papillate regions of the hindgut, where the highest concentrations of bacteria are commonly found. The amplified genes were cloned, sequenced and phylogenetically analysed. The results implied an unexpectedly large diversity of microflora associated with the cuticle of the hindgut. Almost half of the retrieved sequences were found to be less than 80% homologous with any of the known sequences available at DNA data banks. Most of these sequences were clustered in one of three groups, and were clearly distant from the sequences of other bacterial taxa, indicating that they could represent novel bacterial species or even genera. More than two thirds of the sequences were found to be phylogenetically related to sequences from bacteria typically isolated from human and animal intestines, e.g. streptococci, enterococci, and members of the genus Bacteroides. The majority of the remaining sequences were most closely related to typical soil bacteria, e.g. bacilli and pseudomonads. The facts that a large proportion of the retrieved sequences was related to the sequences of bacteria, which are autochthonous to intestinal ecosystems, and that bacteria, specifically attached to the cuticular spines, were observed, indicate that truly autochthonous bacteria may well be present in the hindgut of P. scaber.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2002.tb00950.x
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  • 2
    Electronic Resource
    Electronic Resource
    Hypogean life-style fuelled by oil (1999)
    Springer
    Naturwissenschaften 86 (1999), S. 43-45 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001140050568
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of Zn on the digestive gland epithelium ofPorceffio scaber (Isopoda, Crustacea) (1996)
    Springer
    Pflügers Archiv 431 (1996), S. R247 
    Details
    ISSN: 1432-2013
    Keywords: zinc toxicity ; hepatopancreas ; biomarker ; Isopoda ; Crustacea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of zinc (Zn)-contaminated food on the shape of digestive gland epithelium was studied in the terrestrial isopod,Porcellio scaber. In animals fed with 5,000 μg Zn g−1 dry wt. of food, the epithelium was flattened in the anterior part of the gland tube. In the posterior part of the gland tube, the shape and size of cells did not change drastically, but they had folded apical surface. In animals fed with more Zn-contaminated food (10,000 μg Zn g−1 dry wt.) the epithelium was uniformly flattened and the basal lamina was intensively folded. In both cases, reduction of lipid bodies was evident. The possibility of using the shape of the gland epithelium as a biomarker of toxic chemicals is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346361
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural analysis of the integument during the moult cycle inLigia italica (Crustacea, Isopoda) (1996)
    Springer
    Pflügers Archiv 431 (1996), S. R251 
    Details
    ISSN: 1432-2013
    Keywords: Isopods ; Moult cycle ; Cuticle ; Mineralization ; X-ray microanalysis ; Calcium granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The formation of the cuticle was investigated during moulting of the isopod crustaceanLigia italica. The intermoult cuticle is a four-layered lamellar structure composed of chitin-protein fibrils and mineralized in its upper half. The distribution of calcium carbonate in cuticle during moult cycle was determined by cytochemical methods and X-ray microanalysis. Epi-and exocuticle are secreted during premoult. Calcium is resorbed from the old cuticle and accumulates in the ecdysal gap as calcium granules. Endocuticle is secreted after moult when the mineralization of exocuticle starts. The shape and ultrastructure of epithelial cells change during cuticle secretion and mineralization. Mitochondria, bundles of filaments, calcium granules and large amounts of glycogen accumulate in the apical cytoplasm of cells in premoult animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346363
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  • 5
    Electronic Resource
    Electronic Resource
    Oleospheres of the cave-dwelling shrimp Troglocaris schmidtii: A unique mode of extracellular lipid storage (1992)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 211 (1992), S. 31-39 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cave-dwelling shrimp, Troglocaris schmidtii, has a unique mode of lipid storage. The lipid lies extracellularly in specialized compartments of the hepatopancreas, named oleospheres. The lipid is synthesized in the R-cells of the hepatopancreatic epithelium and accumulates in lipid droplets which fuse to form bigger globules. Mature lipid globules display moderately electron dense centers probably comprising triglycerides, and a broad electron dense boundary presumably consisting of lipoproteins. The globules are discharged into the lumen of the hepatopancreatic tubules by a kind of apocrine secretion. There, they coalesce to form larger masses. Finally, these lipid masses are transported into the oleospheres through a valve-like structure. The continual accumulation of lipid results in a drastic expansion of the oleospheres up to 500 μm in diameter. The absence of food in the digestive tract and the inactivity of the digestive enzyme producing F-cells indicate that digestion is suspended in the period of oleosphere formation. The curious mode of lipid storage in T. schmidtii may represent an adaptation to the extreme environmental conditions of a cave.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052110105
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    Paper (German National Licenses)
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